M1受体参与孤啡肽抑制大鼠顶叶皮层神经元延迟整流钾电流

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (8): 853-857.

M1受体参与孤啡肽抑制大鼠顶叶皮层神经元延迟整流钾电流

杨旭¹,曲丽辉¹,田华²,崔岚巍¹,王鹏¹,宋英莉¹,李玉荣³

1. 哈尔滨医科大学生理教研室
2. 齐齐哈尔医学院药理学教研室
3. 哈尔滨医科大学生理学教研室

收稿日期:2010-05-05 修回日期:2010-10-18 出版日期:2011-08-05 发布日期:2011-07-13
通讯作者: 李玉荣 E-mail:yangxu86110@126.com
基金资助:
国家自然科学基金;高等学校博士点基金

M1-receptor involved in Orphanin FQ-induced the inhibition of delayed rectifier potassium current in rat cerebral parietal cortical neurons

Xu YANG¹,Li-hui QU²,Hua TIAN²,Lan-wei CUI²,Peng WANG²,Li-ying SONG²,Yu-rong LI¹

1. Harbin Medical University
2.

Received:2010-05-05 Revised:2010-10-18 Online:2011-08-05 Published:2011-07-13
Contact: Yu-rong LI E-mail:yangxu86110@126.com

摘要/Abstract

摘要： 目的研究M受体不同亚型对孤啡肽（N/OFQ）抑制大鼠顶叶皮层神经元延迟整流钾电流（IK）作用的影响。方法采用全细胞膜片钳技术，应用M受体不同亚型阻断剂，观察M受体亚型在孤啡肽抑制大鼠顶叶皮层神经元IK中的作用。结果（1）N/OFQ对大鼠顶叶皮层神经元IK有抑制作用，抑制率为（30.8±5.6）%（P<0.01）。（2）M1受体拮抗剂哌伦西平（PIR）可减弱N/OFQ对IK的抑制作用，在指令电压为+60mV时，PIR+N/OFQ组的IK幅度下降了（15.5±2.5）%，与单独给予N/OFQ所引起的IK抑制效应相比，有显著差异（P<0.05）。（3）M3受体拮抗剂4DAMP拮抗N/OFQ对IK的抑制作用不明显。结论 M1受体参与N/OFQ对大鼠顶叶皮层神经元IK的抑制作用。

Abstract: Objective To study the effect of M1 and M3 receptors’ antagonists on the inhibition of delayed rectifier potassium current ( IK ) by nociceptin/orphanin FQ ( N/OFQ ) in acutely dissociated rat cerebral parietal cortical neurons. Methods Using whole-cell patch-clamp technique, recording effects of different subtypes of M receptor on the inhibition of IK by N/OFQ in rat cerebral cortical neurons. Results ( 1 ) N/OFQ inhibited IK in acutely dissociated rat cerebral parietal cortical neurons, and the inhibition rate was ( 30.8±5.6 )% ( P < 0.01 ); ( 2 ) PIR M1 receptor antagonist antagonized the inhibitory effect of N/OFQ on IK and the drop of currents varied from the control value of ( 30.8±5.6 )% to ( 15.5±2.5 )% ( P < 0.05 ); ( 3 ) M3 receptor antagonist 4DAMP has not obvious effects on the inhibitory effect of N/OFQ on IK. Conclusion M1-receptor involved in the inhibition of N/OFQ on IK in acutely dissociated rat cerebral parietal cortical neurons.

中图分类号:

杨旭曲丽辉田华崔岚巍王鹏宋英莉李玉荣. M1受体参与孤啡肽抑制大鼠顶叶皮层神经元延迟整流钾电流 [J]. 基础医学与临床, 2011, 31(8): 853-857.

Xu YANG Li-hui QU Hua TIAN Lan-wei CUI Peng WANG Li-ying SONG Yu-rong LI. M1-receptor involved in Orphanin FQ-induced the inhibition of delayed rectifier potassium current in rat cerebral parietal cortical neurons[J]. Basic & Clinical Medicine, 2011, 31(8): 853-857.