CDK2促进K562细胞早期红系分化

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (5): 561-564.

CDK2促进K562细胞早期红系分化

李均¹,岳瑞华¹,²,沈钧乐¹,²,肖俊¹,²

1. 重庆市急救医疗中心重庆市第四人民医院
2.

收稿日期:2010-12-03 修回日期:2011-02-18 出版日期:2011-05-05 发布日期:2011-05-06
通讯作者: 李均 E-mail:luck_lijun@163.com

CDK2 accelerates early erythroid differentiation of K562 cells

Jun LI¹,Rui-hua YUE³,Jun-le SHEN³,Jun XIAO³

Received:2010-12-03 Revised:2011-02-18 Online:2011-05-05 Published:2011-05-06
Contact: Jun LI E-mail:luck_lijun@163.com

摘要/Abstract

摘要： 目的探讨细胞周期调节蛋白CDK2对K562细胞红系分化的影响。方法分别用CDK2表达质粒和干扰RNA分子转染K562细胞，用Western blot法检测过表达或干扰效率，使用real-time PCR和联苯胺染色法检测K562细胞分化。结果 CDK2在K562细胞红系分化早期呈现表达上升趋势；在K562细胞中过表达CDK2可促进hemin诱导的红系分化；反之，干扰K562内源的CDK2表达会对K562红系分化产生抑制作用。结论 CDK2在K562细胞早期红系分化过程中发挥促进作用。

关键词: CDK2, K562, γ-globin, 红系分化

Abstract: Objective To study the roles of a cell cycle regulator Cyclin-dependent kinase 2 (CDK2) in erythroid differentiation of K562 cells.Methods K562 cells were transfected with the construct expressing CDK2 and siRNAs specifically targeting CDK2, respectively. The effects of over-expression or knocking-down of CDK2 were examined by Western blot. Quantitative RT-PCR was performed to detect the levels ofγ-globin mRNA expression. The benzidine staining assay was used to access the differentiation state of K562 cells. Results CDK2 was up-regulated at the early stage of K562 erythroid differentiation. Over-expression of CDK2 in K562 cells accelerated erythroid differentiation, in contrast, inhibition of CDK2 attenuates globin accumulation in K562 cells.Conclusion CDK2 is required for early erythroid differentiation of K562 cells.

Key words: CDK2, K562, γ-globin, erythroid differentiation

李均岳瑞华沈钧乐肖俊. CDK2促进K562细胞早期红系分化[J]. 基础医学与临床, 2011, 31(5): 561-564.

Jun LI Rui-hua YUE Jun-le SHEN Jun XIAO. CDK2 accelerates early erythroid differentiation of K562 cells[J]. Basic & Clinical Medicine, 2011, 31(5): 561-564.

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