恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3 佐剂的配伍

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (5): 496-502.

恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3 佐剂的配伍

马瑞森,蔺亚辉,张文文,麻明,王恒

中国医学科学院基础医学研究所

收稿日期:2011-02-18 修回日期:2011-03-22 出版日期:2011-05-05 发布日期:2011-05-06
通讯作者: 王恒 E-mail:wanghpumc@gmail.com
基金资助:
国家高技术研究发展（863）计划

Effects and immune mechanism of different adjuvants on polyepitope chimeric vaccine M.RCAg-1&M.RCAg-3

MA Rui-sen ¹,LIN Ya-hui ²,ZHANG Wen-wen ²,MA Ming ²,WANG Heng ¹

1. IBMS, CAMS&PUMC
2.

Received:2011-02-18 Revised:2011-03-22 Online:2011-05-05 Published:2011-05-06
Contact: WANG Heng E-mail:wanghpumc@gmail.com

摘要/Abstract

摘要： 目的评价恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3与不同佐剂最优配伍方式并进行初步的免疫机制研究。方法将多表位疫苗表达纯化后混合4种佐剂（AD、ADL、CpG及CpG&Al）经皮下注射BAL B/c小鼠，免疫3次，每次间隔两周。测定血清中抗M.RCAg-1和M.RCAg-3抗体滴度、IgG分型及血清抗体对抗原单表位识别。间接免疫荧光检测血清对天然疟原虫的识别。结果佐剂ADL和AD分别能显著增强M.RCAg-1（P<0.05）和M.RCAg-3（P<0.05）免疫反应。而佐剂CpG和CpG&Al增强抗原免疫反应的能力相对较弱。与M.RCAg-1、M.RCAg-3和CpG、CpG&Al配伍的组相比，AD和ADL佐剂组的抗体水平和间接免疫荧光反应也都显著增强（P<0.05, P<0.05），抗体对单表位识别显著，抗体IgG分型中IgG1和IgG2a水平较高。结论佐剂ADL和AD的效果显著，可以作为与M.RCAg-1和M.RCAg-3配伍的候选临床应用佐剂。

关键词: 恶性疟原虫, 多表位疫苗, 佐剂配伍

Abstract: Objective To investigate the formulations of Plasmodium falciparum polyepitope chimeric vaccine M.RCAg-1 & M.RCAg-3 with different adjuvants in order to increase their immunogenicities and to find out their basic immune mechanisms. Methods The polyepitope chimeric vaccines formulated with different adjuvants (AD, ADL, CpG, CpG&Al) were injected subcutaneously into mice for three times. The immunization was performed on week 0, 2, 4. The concentration of anti-M.RCAg-1 and anti-M.RCAg-3 IgG, the IgG isotype and the recognition level with M.RCAg-1/M.RCAg-3 single epitopes were detected at ten days after week 4. The recognition of antibodies in serum was tested with indirect immunofluorescence assay (IFA). Results We showed here that ADL and AD can enhance the immunogenicities of M.RCAg-1 and M.RCAg-3 respectively. Compared with another groups, ADL and AD formulated with M.RCAg-1 and M.RCAg-3 respectively can enhance their antibodies recognition with natural parasites greatly. Their antibodies can recognize the single epitopes generally. Conclusion ADL and AD can greatly increase the immunization antibody level of M.RCAg-1 and M.RCAg-3 and their recognition with natural parasites. In conclusion, ADL and AD, as potential adjuvants, paved the way for the clinical use of M.RCAg-1 and M.RCAg-3 respectively.

Key words: Plasmodium falciparum, polyepitope chimeric vaccine, adjuvants formulation

中图分类号:

R531.3

马瑞森蔺亚辉张文文麻明王恒. 恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3 佐剂的配伍[J]. 基础医学与临床, 2011, 31(5): 496-502.

MA Rui-sen LIN Ya-hui ZHANG Wen-wen MA Ming WANG Heng. Effects and immune mechanism of different adjuvants on polyepitope chimeric vaccine M.RCAg-1&M.RCAg-3[J]. Basic & Clinical Medicine, 2011, 31(5): 496-502.