高浓度氧增加未成年大鼠肺血红素加氧酶-1表达

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (2): 124-127.

高浓度氧增加未成年大鼠肺血红素加氧酶-1表达

吴长毅¹,岳峰¹,李民¹,张利萍¹,王军¹,郭向阳²

1. 北京大学第三医院
2. 北京大学第三医院麻醉科

收稿日期:2010-04-19 修回日期:2010-06-25 出版日期:2011-02-05 发布日期:2011-03-14
通讯作者: 郭向阳 E-mail:xyg42@yahoo.com.cn
基金资助:
国家自然科学基金

Hyperoxia increases the expression of lung heme oxygenase-1 in rat pups

WU Chang-yi ¹,YUE Feng ²,LI Min ²,ZHANG Li-ping ²,WANG Jun ²,GUO Xiang-yang ³

1. Peking University Third Hospital
2.
3. Department of Anesthesia, Third Hospital of Peking University

Received:2010-04-19 Revised:2010-06-25 Online:2011-02-05 Published:2011-03-14
Contact: GUO Xiang-yang E-mail:xyg42@yahoo.com.cn

摘要/Abstract

摘要： 目的探讨高浓度氧对未成年大鼠肺血红素加氧酶－1（HO-1）表达的影响。方法将生后 21 d SD 大鼠 40 只随机分为空气组和 12、24、48 及72 h 高氧组，分别置于空气和常压高氧箱（92％～94％ O2）中。检测左肺湿/干重比及肺组织病理学改变，用 RT-PCR 和 Western blot 法分别检测肺 HO-1mRNA 及 HO-1 蛋白表达情况。结果高氧 12 h 组肺 HO-1 mRNA 吸光度积分相对值和高氧 24 h 组 HO-1 蛋白表达水平分别为 0.35 ± 0.043 和 0.455 ± 0.046，较对照组0.263 ± 0.037 和 0.28 ± 0.044 明显升高，且均随高氧暴露时间延长而表达进一步增加（P < 0.05，P < 0.01）。与空气组比较，高氧 48 h 组和高氧 72 h 组左肺湿重/干重及肺损伤评分显著增高（P < 0.05，P < 0.01）。结论高浓度氧可引起未成年大鼠肺组织 HO-1 表达增多。

关键词: 高浓度氧, 血红素加氧酶－1, 肺损伤, 未成年大鼠

Abstract: Objective To investigate the effects of hyperoxia on the expression of lung heme oxygenase-1 (HO-1) in rat pups. Methods 21-d-old male Sprague-Dawley rat pups were randomly divided into 5 groups: room-air control group and 12, 24, 48, 72 hours hyperoxia groups. The rat pups were continuously exposed to oxygen (92%～94%) and room-air respectively. The left lung wet/ dry weight ratio and the histological examination were performed. The expressions of lung HO-1 mRNA and HO-1 protein were also measured by RT-PCR and Western blot, respectively. Results The expressions of lung HO-1 mRNA in 12 hours hyperoxia group (0.35±0.043) and HO-1 protein in 24 hours hyperoxia group (0.455±0.046) were increased as compared to those in the room-air group (0.263±0.037, 0.28±0.044), and the increases were more significant with the longer hyperoxia exposure (P < 0.05, P < 0.01). Compared with the room-air control, the left lung wet/ dry weight ratio and the lung injury scores were significantly increased in 48 hours hyperoxia group and 72 hours hyperoxia group (P < 0.05, P < 0.01). Conclusion Hyperoxia can induce the increase of lung heme oxygenase-1 expression in rat pups.

中图分类号:

吴长毅岳峰李民张利萍王军郭向阳. 高浓度氧增加未成年大鼠肺血红素加氧酶-1表达 [J]. 基础医学与临床, 2011, 31(2): 124-127.

WU Chang-yi YUE Feng LI Min ZHANG Li-ping WANG Jun GUO Xiang-yang. Hyperoxia increases the expression of lung heme oxygenase-1 in rat pups[J]. Basic & Clinical Medicine, 2011, 31(2): 124-127.

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