基础医学与临床 ›› 2010, Vol. 30 ›› Issue (5): 520-523.

• 研究论文 • 上一篇    下一篇

骨髓间充质干细胞向软骨细胞分化过程中miR130a的表达

苏金梅 金晔 曲强 张奉春 唐福林   

  1. 中国医学科学院北京协和医学院北京协和医院 中国医学科学院北京协和医学院北京协和医院 中国医学科学院北京协和医学院北京协和医院 中国医学科学院 北京协和医学院 北京协和医院 风湿免疫科 中国医学科学院北京协和医学院北京协和医院 风湿免疫科
  • 收稿日期:2009-12-23 修回日期:2010-01-06 出版日期:2010-05-05 发布日期:2010-05-05
  • 通讯作者: 唐福林

The expression of microRNA130a during chondrogenic differentiation of rat bone mesenchymal stem cells

Jin-mei SU, Ye JIN, Qiang QU, Feng-chun ZHANG, Fu-lin TANG   

  1. PUMC Hospital, CAMS & PUMC PUMC Hospital, CAMS & PUMC Department of Rheumatology,PUMC Hospital,CAMS&PUMC Department of Rheumatology,PUMC Hospital,CAMS&PUMC
  • Received:2009-12-23 Revised:2010-01-06 Online:2010-05-05 Published:2010-05-05
  • Contact: Fu-lin TANG

摘要: 目的 诱导骨髓间充质干细胞向软骨细胞分化,初步探索miR130a在骨髓间充质干细胞向软骨细胞分化过程中的调控作用。方法 体外TGF-β1诱导骨髓间充质干细胞向软骨细胞分化,免疫荧光法和免疫组化染色鉴定II型胶原,阿辛兰染色鉴定氨基葡聚糖。用Real-time PCR法检测细胞miR130a的表达。结果 骨髓间充质干细胞在TGF-β1诱导下可分化为软骨细胞。培养7天后,诱导培养组细胞miR130a表达的水平显著低于培养前的水平(P<0.05)。结论 骨髓间充质干细胞体外诱导可以分化为软骨细胞,在向软骨细胞分化的早期,miR130a表达降低伴随着软骨细胞的分化,提示与软骨形成的过程有关。

关键词: 骨髓间充质干细胞, 软骨细胞, 分化, microRNA

Abstract: Objective To evaluate the role of microRNA130a during chondrogenic differentiation of rat bone mesenchymal stromal cells (BMSCs). Methods BMSCs were induced to differentiate into chondrocytes by transforming growth factor-β1(TGF-β1) in vitro, immunofluorescence and immunohistochemistry were performed to evaluate MSCs differentiation. Real-time reverse transcription polymerase chain reaction was performed to analyze microRNA130a expression at different time points (before induced culture, 7 days later in induced cultures and 7 days later in non-induced culture). Results We found microRNA130a was down-modulated during chondrogenesis after BMSCs had cultured in the present of TGF-β1 for 7 days(P<0.05). Conclusion These findings show that, during the early stage of BMSC chondrogenic differentiation, mciroRNA130a expression was specifically repressed, suggesting a role in differentiation of rat bone mesenchymal stromal cells.

Key words: bone mesenchymal stem cell, chondrocyte, differentiation, microRNA

中图分类号: