基础医学与临床 ›› 2010, Vol. 30 ›› Issue (2): 185-188.

• 研究论文 • 上一篇    下一篇

刚地弓形虫培养上清抑制肺癌A549细胞系增殖

葛璞 李春莉 冉亚林 叶彬   

  1. 重庆医科大学 重庆医科大学 重钢总医院 重庆医科大学
  • 收稿日期:2009-06-23 修回日期:2009-08-21 出版日期:2010-02-05 发布日期:2010-02-05
  • 通讯作者: 叶彬

Culture Supernatant of Toxoplasma gondii Inhibits The Proliferation of Human Lung Cancer Cell Line A549

Pu GE, Chun-li LI, Ya-lin RAN, Bin YE   

  1. Chongqing Medical University Chongqing Steel Hospital Chongqing Medical University
  • Received:2009-06-23 Revised:2009-08-21 Online:2010-02-05 Published:2010-02-05
  • Contact: Bin YE

摘要: 目的 研究刚地弓形虫(Toxoplasma gondii)培养上清对体外培养的肺癌细胞A549增殖及细胞周期的影响。方法 取对数生长期的A549细胞(浓度为5×104mL-1)分别接种于不同细胞培养板,对照组加入RPMI-1640孵育,试验组加入相同体积不同数量(4×107mL-1、 8×107mL-1、 16×107 mL-1 )弓形虫速殖子培养上清孵育不同时间后,四甲基氮噻唑蓝(MTT)法检测吸光度(A(490)值);PI染色后检测细胞周期;以Westernblot检测细胞周期蛋白cyclinB1、cdc2表达或活性。结果弓形虫培养上清呈时间剂量依赖性抑制A549细胞系增殖,处理组细胞周期在G2/M期产生阻滞; A549细胞系的cyclinB1、cdc2 蛋白表达量下降。结论 刚地弓形虫培养上清能够抑制肺癌A549细胞系增殖并通过调节cyclinB1、cdc2等蛋白表达或活性改变引起肺癌A549细胞系G2/M期阻滞。

关键词: 刚地弓形虫, 人肺癌细胞A549, 细胞周期

Abstract: Objective: To investigate whether there is inhibition of the human lung cancer cell line A549 induced by the culture supernatant of Toxoplasma gondii in vitro and the mechanism of the inhibition. Methods A549 cells 5×104mL-1 were cultured and harvested in the different cell culture plates. The cells were treated for different hours with different concentrations of Toxoplasma gondii culture supernatant (the concentrations of tachyzoites were 4×107mL-1, 8×107mL-1, 16×107mL-1 respectively). Growth inhibition rates were measured with the MTT method;Cell cycle was checked with Flow Cytometer. Western blot was used to detect the leves of cyclinB1、cdc2 of cells. Results The culture supernatants of Toxoplasma gondii could inhibit the proliferation of A549 cells in a time-dose dependent manner. Cell cycle was significantly stopped at G2/M phase by the culture supernatants with FCM technology. The culture supernatant of Toxoplasma gondii reduced the expressions of gene cyclinB1and cdc2 of A549 cells. Conclusion The culture supernatant of Toxoplasma gondii may inhibit A549 cell and arrest the cell cycle of A549 cells mainly by regulating the expression of gene cyclinB1 and cdc2

Key words: Toxoplasma gondii, Lung cancer cell A549 cell, cell cycle