基础医学与临床 ›› 2010, Vol. 30 ›› Issue (2): 159-164.

• 研究论文 • 上一篇    下一篇

PLOD2参与人皮肤纤维化的形成

朱春晖 罗忠金   

  1. 南昌大学第一附属医院
  • 收稿日期:2009-02-11 修回日期:2009-05-25 出版日期:2010-02-05 发布日期:2010-02-05
  • 通讯作者: 朱春晖

PLOD2 is Involved in Human Skin Fibrosis

Chun-hui ZHU, Zhong-jin LUO   

  1. the First Affiliated Hospital of Nanchang University
  • Received:2009-02-11 Revised:2009-05-25 Online:2010-02-05 Published:2010-02-05
  • Contact: Chun-hui ZHU,

摘要: 目的 探讨瘢痕组织中赖氨酸羟化酶2基因(PLOD2)的表达与I型胶原(COLIα1)形成之间的相关性。方法用RT-PCR及Western blot检测瘢痕组织和正常皮肤组织中PLOD2及 COLIα1 mRNA 的表达及I型胶原蛋白的含量;设计并合成三种PLOD2 ASODN(分别针对PLOD2翻译起始部、第一与第二外显子连接部、终止密码部),分别转染入NIH/3T3小鼠成纤维细胞,用RT-PCR及Western blot检测细胞PLOD2和 COLIα1mRNA表达及I型胶原的含量。结果 瘢痕组织中PLOD2的表达和I型胶原的含量明显高于正常皮肤组织(P<0.05);3种ASODN对成纤维细胞PLOD2 mRNA的表达及Ι型胶原的形成均产生抑制作用(P<0.05),其中针对PLOD2翻译起始部的ASODN抑制率最高(P<0.001);在瘢痕组织与正常皮肤组织之间,以及反义ODN治疗各组之间,COLIα1的表达无差异。结论 PLOD2高表达与I型胶原形成密切相关,PLOD2 ASODN阻止Ι型胶原形成的作用位于COLIα1 mRNA转录后水平。

关键词: 寡脱氧核苷酸, 赖氨酸羟化酶2基因, Ⅰ型胶原

Abstract: Objective To discuss the relationship between the expression of PLOD2 and the formation of collagen I in the scar. Methods To detect the mRNA expression of PLOD2 and COLIα1 in the scar and normal skin tissue , as well as the content of COLIα1 by RT-PCR and western blot; design and synthesis three kinds of PLOD2 ASODN targeted against the translational initiation , the joint of the first and the second exons and termination codon respectively,transfected into NIH/3T3 mouse fibroblast cell and detect the mRNA expression of COLIα1 and formation of collagen I by RT-PCR and western blot .Results : the expression of PLOD2 in the scar tissue is significantly higher than that in the normal tissue(P<0.05), three kinds of ASODNs exert inhibitory effect on the mRNA expression of COLIα1 and formation of collagen I in fibroblast(P<0.05), during which the repressive efficiency of ASODN targeted against the translational initiation is the highest(P<0.001); while the mRNA expression level of COLIα1 has no statistical difference in the scar tissue , normal tissue and the groups treated with three kinds of ASODN. Discussions the high expression of PLOD2 is tightly related to the formation of collagen I, PLOD2 ASODN can suppress the formation of collagen I post-transcriptionally.

Key words: oligodeoxyribonucleotide, procollage-lysine 2-oxoglutarate 5-dioxygenase 2, collagenⅠ