基础医学与临床 ›› 2009, Vol. 29 ›› Issue (6): 603-606.

• 研究论文 • 上一篇    下一篇

无创性延迟肢体缺血预适应减轻大鼠脑缺血/再灌注损伤

罗琼 袁恒杰 吴艳娜 康毅 高卫真 刘艳霞 娄建石   

  1. 天津医科大学药理教研室 天津医科大学 天津医科大学药理教研室 天津医科大学药理教研室 天津医科大学药理教研室 天津医科大学药理教研室 天津医科大学药理教研室
  • 收稿日期:2008-09-16 修回日期:2008-12-22 出版日期:2009-06-25 发布日期:2009-06-25
  • 通讯作者: 罗琼

Noninvasive delayed limb ischemic preconditioning decreases cerebral ischemia-reperfusion injury in rats

Qiong LUO, Heng-jie YUAN, Yan-na WU, Yi KANG, Wei-zhen GAO, Yan-xia LIU, Jian-shi LOU   

  1. Department of Pharmacology, Tianjin Medical University Department of Pharmacology, Tianjin Medical University
  • Received:2008-09-16 Revised:2008-12-22 Online:2009-06-25 Published:2009-06-25
  • Contact: Qiong LUO,

摘要: 目的 研究无创性延迟肢体缺血预适应(NDLIP)对大鼠脑缺血/再灌注后氧化损伤的保护作用及其作用机制。方法 连续3d,每天1次3个循环大鼠左后肢无创性5min缺血、5min再灌注,建立NDLIP模型。实验分4组:假手术组、缺血再灌(I/R)组、早期脑缺血预适应+I/R(ECIP+I/R)组及NDLIP+I/R组。进行神经行为评分,检测脑梗死范围,测定再灌末脑组织中海马和皮层超氧化物歧化酶(SOD)以及丙二醛(MDA)含量。结果 与I/R组相比,ECIP+I/R组及NDLIP+I/R组缺血1h,再灌24h后评分有非常显著的降低(P<0.01);脑梗死范围显著减小(P<0.01)。与I/R组比较,ECIP+I/R 组和NDLIP+I/R组的海马和皮层的T-SOD和Mn-SOD活力均升高(P<0.01); MDA含量明显减少(P<0.01)。结论 对脑缺血/再灌注后的氧化损伤,NDLIP具有与ECIP程度相当的保护作用。

关键词: 肢体, 远端缺血预适应, 脑缺血/再灌注损伤, 超氧化物歧化酶, 丙二醛

Abstract: Objective To study the protective effects of noninvasive delayed limb ischemic preconditioning against cerebral ischemia-reperfusion oxidative injury in rats. Method Wistar rats were divided randomly into sham operation(sham), ischemia-reperfusion(I/R), early cerebral ischemic preconditioning and ischemia-reperfusion(ECIP+I/R), and noninvasive delayed limb ischemic preconditioning and ischemia-reperfusion(NDLIP+I/R) groups. Rats in NDLIP+I/R group were subjected to three cycles of five minutes ischemia/five minutes reperfusion on the left hind limb, once a day for three consecutive days. On the forth day, all rats were subjected to cerebral ischemia/reperfusion injury. Rats in ECIP+I/R group were preconditioned before ischemia. Neurological score and cerebral infarct size(IS) were examined after ischemia 1h and reperfusion 24h. Activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA) were measured. Results Compared with I/R group, ECIP+I/R and NDLIP+I/R could reduce neurological score significantly after ischemia 1h and reperfusion 24h(P<0.01). Compared with I/R group, ECIP+I/R and NDLIP+I/R could reduce IS and concentration of MDA, increase total SOD(T-SOD)and Mn-SOD activity in hippocamppus and cortex(P<0.01). Among all these oxidative and antioxidative substancese, there was no significance between ECIP+I/R and NDLIP+I/R. Conclusion The protective effects of NDLIP against cerebral ischemia-reperfusion injury are similar to those of ECIP, the protective effects are associated with the increase in antioxidative ability of brain.

Key words: Limb, Remote ischemic preconditioning, Cerebral ischemia-reperfusion injury, Superoxide dismutase, Malondialdehyde