基础医学与临床 ›› 2009, Vol. 29 ›› Issue (5): 488-494.

• 研究论文 • 上一篇    下一篇

广西三个少数民族17个Y-STR位点的遗传多态性

梁祚仁 刘长晖 刘超   

  1. 广州医学院 广州市刑事科学技术研究所
  • 收稿日期:2008-06-24 修回日期:2008-07-28 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 刘超

Polymorphism of 17Y-STR loci in three minority populations in Guangxi of China

Zuo-ren LIANG, Chang-hui LIU, Chao LIU   

  1. Guangzhou Medical College Guangzhou Criminal Science and Technology Institute
  • Received:2008-06-24 Revised:2008-07-28 Online:2009-05-25 Published:2009-05-25
  • Contact: Chao LIU

摘要: 目的 研究广西侗、苗、壮3个少数民族17个Y-STR基因座的多态信息及其在法医学应用价值。方法 少数民族口腔拭子用磁珠法提取DNA后用Yfiler复合扩增试剂盒扩增,3100XL型遗传分析仪进行基因分型。计算基因频率,多样性和遗传距离。结果 获得3个少数民族309名男性17个Y-STR基因座的等位基因频率分布资料,3个少数民族单倍型共观察到275种。单倍型多样性:侗族为0.9972,苗族为0.9966,壮族为0.9964;遗传距离中苗族跟侗族的遗传距离最近。结论 由17个Y-STR位点组成的单倍型多样性在种族鉴别,父权鉴定和群体遗传学等方面有很高的应用价值。

关键词: Y染色体, 单倍型, 遗传多态性, 遗传距离, STR

Abstract: Objective  To investigate the polymorphism of seventeen Y chromosomal short tandem repeat (STR) loci in dong, miao and zhuang nationality of Guangxi of China,and then evaluate their forensic application.Method the DNA was extracted from oral swab of minority population with magnetic beads method and then seventeen Y-STR loci were amplified with AmpFlSTR? Yfiler? PCR Amplification Kit System . The PCR products were detected with 3l30XL Genetic Analyzer. The allele frequencies,diversities and genetic distance were calculated .Results The information of allele distribution and gene diversity of the 17 Y-STR loci were obtained from three minority population.275 haplotypes were found in three minority population. The haplotype diversity of dong nationality is 0.9972,Miao is 0.9966,zhuang is 0.9964.The genetic distance between dong and miao nationality is most close. Conclusion The haplotype diversity of 17 Y-STR loci is so effective in racial identification, paternity identification and human population genetics.

Key words: Y chromosome, haplotype, genetic polymorphism, genetic distanc, STR