FKN对人外周血单个核细胞PKC-ERK1/2活化和TNF-α表达的影响

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (2): 148-151.

FKN对人外周血单个核细胞PKC-ERK1/2活化和TNF-α表达的影响

雷明明孙健吴哲杨春艳陈玉花

吉林大学第二医院心血管内科吉林大学第二医院心血管内科吉林大学第二医院心血管内科吉林大学第二医院心血管内科

收稿日期:2008-04-30 修回日期:2008-06-06 出版日期:2009-02-25 发布日期:2009-02-25
通讯作者: 孙健

Effects of FKN on PKC-ERK1/2 activation and TNF-α expression in human peripheral blood monocytes

Ming-ming LEI, Jian SUN, Zhe WU, Chun-yan YANG, Yu-hua CHEN

Department of Cardiovascular Medicine,the Second Hospital of Jilin University Department of Cardiovascular Medicine,the Second Hospital of Jilin University Department of Cardiovascular Medicine,the Second Hospital of Jilin University Department of Cardiovascular Medicine,the Second Hospital of Jilin University

Received:2008-04-30 Revised:2008-06-06 Online:2009-02-25 Published:2009-02-25
Contact: Jian SUN,

摘要/Abstract

摘要： 目的探索FKN-CX3CR1在单个核细胞中可能存在的信号传导途径及促进动脉粥样硬化形成的机制，并探讨蛋白激酶C（PKC）在其中作用。方法（1）用Ficoll密度梯度离心法分离抗凝人外周血单个核细胞。（2）将每份提取的单个核细胞分为4组：对照组、FKN组、Ro31-8220（PKC特异性阻断剂）和PD98059（ERK1/2特异性阻断剂）组。（3）用Western blot法检测单个核细胞中磷酸化ERK1/2表达。（4）用ELISA法检测培养液中TNF-α的表达。结果（1）FKN组磷酸化的ERK1/2和TNF－α表达较对照组显著增多（P<0.05）。（2）Ro31-8220组磷酸化的ERK1/2和TNF－α表达较FKN组显著减少（P<0.05）。结论 FKN－CX3CR1可能通过PKC/ERK途径诱导单个核细胞TNF-α的表达，最终促进动脉粥样硬化的形成和进展。

Abstract: Objective To explore one of the possible signal transduction pathways of FKN/CX3CR1 in human peripheral blood monocytes (PBMC) and the mechanism of FKN in promoting atherosclerosis,the function of protein kinase C is also studied.Methods （1）Human peripheral blood monocytes were isolated from fresh blood of healthy volunteers by Ficoll-Paque gradient centrifugation.（2）Divided the PBMC into four groups:control,FKN,Ro31-8220 and PD98059 groups.（3）Measured the ERK 1/2 expression of monocytes from each group by Western blot.（4）Collected the supernatant of monocytes from each group ,determined the expression of TNF-α by enzyme-linked immunosorbent assay(ELISA).Results （1）The expression of ERK1/2 and TNF－α from FKN group was significantly higher compared with the control and TNF－α from FKN group was significantly higher compared with the control group（P<0.05）.（2）The expression of ERK1/2 and TNF－α from Ro31-8220 group was decreased compared with the FKN group（P<0.05）.Conclusions FKN－CX3CR1 induces the expression of TNF－α via PKC/ERK signal pathway which contributines to the development and progression of atherosclerosis.

雷明明孙健吴哲杨春艳陈玉花. FKN对人外周血单个核细胞PKC-ERK1/2活化和TNF-α表达的影响[J]. 基础医学与临床, 2009, 29(2): 148-151.

Ming-ming LEI; Jian SUN; Zhe WU; Chun-yan YANG; Yu-hua CHEN. Effects of FKN on PKC-ERK1/2 activation and TNF-α expression in human peripheral blood monocytes[J]. Basic & Clinical Medicine, 2009, 29(2): 148-151.