基础医学与临床 ›› 2009, Vol. 29 ›› Issue (2): 144-147.

• 研究论文 • 上一篇    下一篇

NSCs-HA-NT-3复合物移植修复兔面神经损伤

张涵 魏跃腾 孙崇然 李晋 黄华 崔福斋 安沂华   

  1. 首都医科大学 北京市神经外科研究所 首都医科大学 北京市神经外科研究所
  • 收稿日期:2008-01-08 修回日期:2008-03-27 出版日期:2009-02-25 发布日期:2009-02-25
  • 通讯作者: 安沂华

Repairing on Rabbit Facial Nerve by the Transplanted of NSCs- HA-NT-3 Complex

Han ZHANG, Yue-teng WEI, Chong-ran SUN, Jin LI, Hua HUANG, Fu-zhai CUI, Yi-hua AN   

  1. Capital Medical University,Beijing Neurosurgical Institute Capital Medical University,Beijing Neurosurgical Institute
  • Received:2008-01-08 Revised:2008-03-27 Online:2009-02-25 Published:2009-02-25
  • Contact: Yi-hua AN

摘要: 目的 观察NSCs-HA-Col支架-NT-3复合物对兔面神经完全横断损伤修复效果。方法 新西兰大耳白兔41只,随机分为6组,E组为NSCs-HA支架-NT-3联合移植组。电生理于术前、术后1、4、8和12周记录肌电图的潜伏期、阈值、振幅。术后12周取桥接处神经,行BrdU及S100免疫组化染色,半薄切片及图像分析,透射电镜观察再生情况。 结果 E组术后12周可见较多BrdU阳性细胞,电生理检测与正常对照无显著性差异,桥接处纵断面呈现连续或不连续波浪状;横断面髓鞘结构与正常对照组的较为相似。结论 NSCs-HA支架-NT-3复合物可以显著提高面神经损伤修复的效果。

关键词: 神经干细胞, 组织工程学, 修复

Abstract: Objective This experiment explored the possibility of repairing injured facial nerve with tissue engineering technology and neural stem cells(NSCs). The complex consisted of NSCs, scaffold and NT-3. NSCs were immature cells with the potential of self-renewal and multiple differentiation to neurons and glial cells. The scaffold with porous surface was made of hyaluronic acid and collagen (HA-Col gel) which degenerate in vivo after transplantation. NT-3 is the signal proved effective in promoting neurons survival in vitro. Methods NSCs of S-D rat were co-cultured with scaffold and NT-3 in vitro. The two stumps of disconnected facial nerve of rabbit were re-connected with the complex. Electrophysiology and morphology tests were used to examine the functional and morphological changes.Results NSCs adhered to the HA-Col gel and survived. Injured facial nerve fixed by NSCs-HA-Col gel-NT-3 complex showed significant improvement in function and anatomical structure. Conclusion The result demonstrated that combinative implant of NSCs, HA-Col gel and NT-3 could promote the regeneration of injured facial nerve.

Key words: neural stem cells, tissue engineering, regeneration