关键词: 血管内皮细胞生长因子, Survivin, 结肠癌, 诱导

Abstract: Objective To investigate the effects of VEGF-induced expression of Survivin in colon cancer cells (LOVO, HCT116) and human umbilical vein endothelial cells (HUVECs). Methods VEGF was used to stimulate the LOVO, HCT116, HUVECs. We used RT-PCR and Western blot to estimate the expression of Survivin in different cells, and immunocytochemistry to investigate the expression level and site of Survivin protein. Results Survivin was expressed highly in the cytoplasm and was upregulated with the induction of VEGF. In HUVECs, Survivin expression was not obvious but significantly upregulated with the inductioin of VEGF in the cytoplasm and nucleus. Conclusion The expression of Survivin mRNA and protein are upregulated by VEGF stimulation. The level and distribution of Survivin are different in tumor cell lines and non-tumorgenicity cells.

Key words: vascular endothelial growth factor, Survivin, colon cancer, induce