基础医学与临床 ›› 2009, Vol. 29 ›› Issue (11): 1166-1169.

• 研究论文 • 上一篇    下一篇

中国儿童A族链球菌感染菌株emm分型及超抗原基因谱分布

马耀玲 杨永弘 俞桑洁 姚开虎 袁林 沈叙庄   

  1. 首都医科大学 北京儿童医院 儿科研究所 首都医科大学 北京儿童医院 儿科研究所 首都医科大学 北京儿童医院 儿科研究所
  • 收稿日期:2008-10-17 修回日期:2009-03-03 出版日期:2009-11-20 发布日期:2009-11-20
  • 通讯作者: 沈叙庄

emm types and superantigen analysis of streptococcus pyogenes isolated from Chinese children

Yao-ling MA, Yong-hong YANG, Sang-jie YU, Kai-hu YAO, Lin YUAN, Xu-zhuang SHEN   

  1. Beijing Children's Hospital Affiliated to University of Medical Science Beijing Children's Hospital Affiliated to University of Medical Science
  • Received:2008-10-17 Revised:2009-03-03 Online:2009-11-20 Published:2009-11-20
  • Contact: Xu-zhuang SHEN

摘要: 目的 研究我国儿科A族β溶血性链球菌感染菌株emm分型及其超抗原在我国的分布情况。方法 用聚合酶链反应(PCR)联合测序对来自中国儿科2005年-2006年222株A族β溶血性链球菌感染的临床菌株进行emm分型;PCR检测speA, speC, speH, speI, speG, speJ, ssa和 SMEZ超抗原基因的分布情况。结果 共检测到9个emm型,emm12为主占55.86%(124/222),其次emm1占39.64%(88/222);78.39%的GAS分离株携带有6个或6个以上的超抗原基因,emm型和超抗原基因谱之间存在密切联系。结论 中国儿科GAS感染流行菌株具有emm型分布集中和超抗原基因携带率高的特点,不同的emm分型有不同的超抗原基因谱,这将为我国GAS疫苗的研究提供重要的分子流行病学依据。

关键词: A群β溶血性链球菌, emm基因分型, 超抗原

Abstract: Objective To investigate the distribution of the emm types and superantigens of group A streptococcal (GAS) isolated from Chinese children. Method 222 GAS isolates obtained from five Children's Hospital of China during 2005-2006 were studied, emm types were performed by PCR and sequencing. And the eight superantigen (SAg) genes (speA, speC, speH, speI, speG, speJ, ssa, and SMEZ) were detected by PCR. Results Nine emm types were determined,of which emm12 (55.86%) and emm1 (39.64%) were the most prevalent types. The GAS isolates carried six or more than six SAg genes were 78.39% of all the isolates in this study. The SAg gene profiles were closely associated with the emm type. Conclusion This study showed that the distribution of the emm types of S. pyogenes isolated from Chinese children were intensively. The SAg genes appeared to be associated with the emm type, thereby aiding in vaccine development in China.

Key words: Group A streptococcus, emm gene sequence, superantigen