老年大鼠组织mRNA分析中内参基因的选择

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (1): 82-84.

老年大鼠组织mRNA分析中内参基因的选择

叶文静赵晓民侯配强蔡洪信夏作理

泰山医学院生命科学研究所

收稿日期:2007-12-25 修回日期:2008-07-17 出版日期:2009-01-25 发布日期:2009-01-25

Reference Genes Selection in mRNA Analysis for Aging Rat Tissues

Wen-jing YE, Xiao-min ZHAO, Pei-qiang HOU, Hong-xin CAI, Zuo-li XIA

Life Science Research Institute of Taishan Medical College

Received:2007-12-25 Revised:2008-07-17 Online:2009-01-25 Published:2009-01-25

摘要/Abstract

摘要： 目的探讨用于老年大鼠组织基因mRNA表达分析的内参基因选择。方法用实时反转录PCR技术评价5个管家基因3-磷酸甘油醛脱氢酶（G3pd）、?-肌动蛋白 (ACTB)、H3组氨酸3B（H3f3b）、酸性核糖体磷蛋白P0（Arbp）和18S核糖体RNA (18S)的表达水平。结果老年大鼠肾组织中表达最稳定的是管家基因ACTB，心脏和肺组织中表达最稳定的是G3pd；而Arbp在不同组织之间的表达差异最小。结论老年大鼠组织间基因表达mRNA分析应使用至少两个参照基因：一个是核糖体基因18S，另一个适合的内参基因是Arbp。

关键词: 管家基因, 内参基因, 实时荧光RT-PCR, mRNA

Abstract: Objective To investigate the proper inner control genes suitable for mRNA expression level comparison of aging rat tissues. Methods Real-time reverse transcription PCR was used to examine in aging rat tissues the expression level of G3pd（glyceraldehyde-3-phosphate dehydrogenase）,ACTB(?-actin),H3f3b（H3 histone, family 3B）,Arbp（acidic ribosomal phosphoprotein P0）and 18S(18S ribosomal RNA ).Results The most stably expressed housekeeping gene in aging rat kidney was ACTB, as for heart and lung G3pd showed the minimum variation; Arbp expression was the most stable among different tissues. Conclusion For aging rat intra-tissue mRNA normalization at least two housekeeping genes should be used: one is the ribosomal RNA gene 18S and the other suitable control gene is Arbp.

Key words: reference genes, HKG, mRNA normalization, real-time RT-PCR

叶文静赵晓民侯配强蔡洪信夏作理. 老年大鼠组织mRNA分析中内参基因的选择[J]. 基础医学与临床, 2009, 29(1): 82-84.

Wen-jing YE; Xiao-min ZHAO; Pei-qiang HOU; Hong-xin CAI; Zuo-li XIA. Reference Genes Selection in mRNA Analysis for Aging Rat Tissues[J]. Basic & Clinical Medicine, 2009, 29(1): 82-84.

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