地塞米松抑制哮喘小鼠肺RANTES的表达

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (9): 978-981.

地塞米松抑制哮喘小鼠肺RANTES的表达

张业清朱述阳吕丽丽朱启勇朱佳

江苏省中西医结合医院徐州医学院附属医院，呼吸科徐州医学院附属医院，呼吸科江苏省中西医结合医院呼吸内科江苏省中西医结合医院呼吸内科

收稿日期:2007-10-31 修回日期:1900-01-01 出版日期:2008-09-25 发布日期:2008-09-25
通讯作者: 张业清

Dexamethasone inhibited the expression of RANTES in the lungs of asthmatic mice

Ye-qing ZHANG, Shu-yang ZHU, Li-li LV, Qi-nan ZHU, Jia ZHU

Jiangsu Provincial Hopital of Integrated Traditional and Western Medicine

Received:2007-10-31 Revised:1900-01-01 Online:2008-09-25 Published:2008-09-25
Contact: Ye-qing ZHANG,

摘要/Abstract

摘要： 目的研究地塞米松对哮喘小鼠调节激活正常T细胞表达和分泌细胞因子（RANTES）蛋白和mRNA表达的影响。方法将30只雌性BALB/c小鼠随机分为3组（每组10只）：对照组、哮喘组、激素干预组，收集支气管肺泡灌洗液（BALF），行白细胞和嗜酸粒细胞（EOS）计数；酶联免疫吸附法（ELISA）测定BALF和血清中RANTES含量；肺组织切片HE染色，光镜下计数细胞总数和EOS百分比；部分行免疫组化染色检测肺组织RANTES蛋白；用原位杂交法检测肺组织RANTES mRNA表达。结果哮喘组白细胞总数、EOS百分比、RANTES浓度明显升高（P<0.01）,干预组明显低于哮喘组（P<0.01）；哮喘组肺组织RANTES蛋白及mRNA表达显著高于对照组（P<0.01），主要表达于上皮细胞；干预组肺组织RANTES蛋白及mRNA表达显著低于哮喘组（P<0.01）。结论地塞米松可抑制RANTES表达和活性而发挥抗EOS炎症作用，这可能是其控制哮喘发病的重要机制之一。

Abstract: Objective :To study the effect of dexamethasone on the protein and mRNA expression of RANTES in asthmatic mice. Methods: Thirty female BALB/c mice were randomly divided into three groups(10 mice in each group) : control group\asthmatic group \dexamethasone group . Bronchoalveolar lavage fliud (BALF) were collected for counting cells and the percentage of eosinophils (EOS); RANTES concentrations in BALF and serum were measured by ELISA.The pathologic changes of bronchi and the left lung tissue were evaluated and stained with hematoxylin and eosin(HE), Immunohistochemistry was used to measure the level of RANTES protein in the lung tissues of asthmatic mice.In situ hybridization was used to measure the level of RANTES mRNA in the lung tissues of asthmatic mice.Results :The total cells、percentage of EOS、concentrations of RANTES in BALF and serum in asthmatic group was significantly different from control group (P<0.01); Dexamethasone group were significantly different from asthmatic group (P<0.01). The expressions of RANTES protein and RANTES mRNA around the lung tissue of asthmatic group were significantly elevated than those of control group (P<0.01), the epithelial cell was the chief expression cell, the expressions of RANTES protein and RANTES mRNA around the lung tissue of dexamethasone group were significantly lower than those of asthmatic group (P <0.01).Conclusion: Dexamethasone demonstrates anti-inflammatory effects partly by downregulating the expressions of RANTES protein and RANTES mRNA.

张业清朱述阳吕丽丽朱启勇朱佳. 地塞米松抑制哮喘小鼠肺RANTES的表达[J]. 基础医学与临床, 2008, 28(9): 978-981.

Ye-qing ZHANG; Shu-yang ZHU; Li-li LV; Qi-nan ZHU; Jia ZHU. Dexamethasone inhibited the expression of RANTES in the lungs of asthmatic mice[J]. Basic & Clinical Medicine, 2008, 28(9): 978-981.