Abstract: Objective To explore the effects of tamoxifen on the proliferation of SHG-44 glioma cells and the currents of sodium channel. Methods The cellular activity was detected by MTT test.The alteration of cellular proliferation and apoptosis was dectected by flow cytometer. Whole-cell patch clamp technique was used to record the Na currents.Results After treatment with tamoxifen,the cells became aging and shedding.The total sum of them decreased.The cells in G2/M cell cycle was more than that in control and the apoptosis ration increased. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line.This block effect was dose dependent and voltage dependent.When the holding potential was 0mV, 8μmol/L tamoxifen could block this currents 69%.The half inhibition concentration(IC50) was 5.54μmol/L. Conclusion Tamoxifen can inhibit SHG-44 glioma cells proliferation.The inhibion of sodium channel may be one of its mechanisms.