Abstract: Cancer stem cells are characterized by self-renewal and multi-differentiation potential, which play an important role in initiation, progression and metastasis of tumor. Interest in cancer stem cells is rapidly increasing, and the existence of such cells in various of tumors has been verified during the recent years. The progress of ovarian cancer stem cells research is reviewed in this article, which suggests that better solutions on isolation and identification of ovarian cancer stem cells are critical tasks currently, and primary cells culture may play a key role in these issues.