CCR5介导6T-CEM 细胞穿过人脑微血管内皮细胞单层能力分析

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (7): 702-706.

CCR5介导6T-CEM 细胞穿过人脑微血管内皮细胞单层能力分析

马怡然尚德淑赵伟东方文刚陈誉华

中国医科大学基础医学院发育生物学教研室中国医科大学基础医学院发育生物学教研室中国医科大学基础医学院发育生物学教研室中国医科大学基础医学院发育生物学教研室中国医科大学基础医学院发育生物学教研室

收稿日期:2007-06-14 修回日期:2007-09-21 出版日期:2008-07-25 发布日期:2008-07-25
通讯作者: 马怡然

CCR5 promotes the migration of 6T-CEM through Human Brain Microvascular Endothelial Cells Monolayer

Yi-ran MA, De-shu SHANG, Wei-dong ZHAO, Wen-gang FANG, Yu-hua CHEN

Received:2007-06-14 Revised:2007-09-21 Online:2008-07-25 Published:2008-07-25
Contact: Yi-ran MA,

摘要/Abstract

摘要： 摘要：目的我们的研究已表明AD病人T淋巴细胞穿过人脑微血管内皮细胞(HBMEC)单层能力高于同年龄正常人与其过表达MIP-1α密切相关，为了分析AD病人T淋巴细胞穿过HBMEC单层机制，选用高表达MIP-1α的人淋巴细胞系6T-CEM作为模式细胞对6T-CEM穿过人脑微血管内皮细胞单层进行探讨。方法通过应用免疫荧光和蛋白免疫印迹技术，真核表达载体的构建与细胞转染技术，以及跨内皮迁移和抗体封闭实验的进行，集中探讨了HBMEC 膜受体CCR5在6T-CEM细胞穿过HBMEC单层过程中作用。结果在6T-CEM 细胞与HBMEC 单层单独孵育过程中，引起HBMEC 膜受体CCR5 表达变化；HBMEC膜受体CCR5的高表达使6T-CEM细胞穿过HBMEC单层能力增强。结论 HBMEC膜受体CCR5参与了6T-CEM细胞穿过HBMEC单层过程。

关键词: 趋化因子受体5, 人T淋巴细胞白血病细胞系, 人脑微血管内皮细胞

Abstract: Abstract: Objective: We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) in peripheral T lymphocytes of AD patients than age-matched is associated with a significantly higher macrophage inflammatory protein-1α (MIP-1α) expression of T lymphocyte. In order to explore the mechanism of the transendothelial migration of T lymphocytes further, we choose 6T-CEM cells expressing MIP-1α as a model cell line of AD patients’ T lymphocytes to investigate this mechanism. Methods :Immunofluorescence, western blot, plasmid construction and transfection, transendothelial migration and endothelial permeability assay are used to explore the progress of the transendothelial migration of T cells. Results: The expressions of CCR5 on HBMECs which incubated with 6T-CEM cells were detected. The induced expressions of CCR5 can enhance the migration of 6T-CEM cells through HBMEC monolayer. Conclusion: CCR5 on HBMECs might be involved in the transendothelial migration of T lymphocytes .

Key words: CCR5, 6T-CEM cells, HBMEC

马怡然尚德淑赵伟东方文刚陈誉华. CCR5介导6T-CEM 细胞穿过人脑微血管内皮细胞单层能力分析[J]. 基础医学与临床, 2008, 28(7): 702-706.

Yi-ran MA; De-shu SHANG; Wei-dong ZHAO; Wen-gang FANG; Yu-hua CHEN. CCR5 promotes the migration of 6T-CEM through Human Brain Microvascular Endothelial Cells Monolayer[J]. Basic & Clinical Medicine, 2008, 28(7): 702-706.