基础医学与临床 ›› 2008, Vol. 28 ›› Issue (6): 610-613.

• 研究论文 • 上一篇    下一篇

肝癌患者血清和癌组织中锌指蛋白216表达增加

顾香芳 余德才   

  1. 南京大学医学院附属鼓楼医院1号楼三层医学检验中心科研部 南京大学医学院附属鼓楼医院1号楼三层医学检验中心科研部
  • 收稿日期:2007-07-19 修回日期:2007-10-30 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 顾香芳

Zinc finger protein 216 up-expressed in hepatocellular carcinoma tissue and sera

Xiang-fang GU, De-cai YU   

  • Received:2007-07-19 Revised:2007-10-30 Online:2008-06-25 Published:2008-06-25
  • Contact: Xiang-fang GU,

摘要: 目的 寻找肝癌相关抗原。方法 应用SEREX技术对肝癌组织cDNA文库进行血清学筛选,获得人类锌指蛋白ZNF216。利用原核表达和亲和层析技术获得ZNF216的6*His融合蛋白,建立间接ELISA方法,检测血清中产生的ZNF216抗体水平;利用RT-PCR技术检测肝癌患者肝癌组织及癌旁组织内ZNF216mRNA的水平。结果 肝癌患者血清中产生的抗ZNF216抗体水平高于正常个体,ZNF216mRNA在肝癌组织内的表达明显高于癌旁组织。结论ZNF216在肝癌病人血清及癌组织内的过表达可能参与肝癌的发生,但还需要进一步的研究证明。

Abstract: Objective To find antigens related with hepatocellular carcinoma (HCC). Methods Human Zinc Finger Protein 216 (ZNF216) was identified by SEREX(serological analysis of recombinant cDNA expression libraries, SEREX). The 6*His fusion protein of ZNF216 was obtained by synthesis bacterially and purified with a nickel affinity chromatography column. The level of anti-ZNF216 antibody in the sera was detected by ELISA,and ZNF216 mRNA level in HCC tissue and its peripheral normal tissue was detected by RT-PCR. Results The level of anti-ZNF216 antibody in the sera of HCC patients was higher than healthy individuals. ZNF216 mRNA level in HCC tissue is evidently higher than peripheral normal tissue. Conclusion The over-expression of ZNF216 in the HCC sera and tissue indicates that ZNF216 perhaps plays a role in HCC, but still needing more evidence.