大鼠骨髓基质细胞在脑内的迁移和分化

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (5): 432-435.

大鼠骨髓基质细胞在脑内的迁移和分化

段春礼姬曼孙晓红赵春礼杨慧

首都医科大学神经科学研究所首都医科大学神经科学研究所首都医科大学神经科学研究所首都医科大学神经科学研究所首都医科大学北京神经科学研究所

收稿日期:2007-03-14 修回日期:2007-07-23 出版日期:2008-05-25 发布日期:2008-05-25
通讯作者: 杨慧

Migration and neural differentiation potential of rat bone marrow stromal cells

Chun-li DUAN, Man JI, Xiao-hong SUN, Chun-li ZHAO, Hui YANG

Beijing Institute for Neuroscience, Capital Medical University Beijing Institute for Neuroscience, Capital Medical University

Received:2007-03-14 Revised:2007-07-23 Online:2008-05-25 Published:2008-05-25
Contact: Hui YANG

摘要/Abstract

摘要： 目的研究骨髓基质细胞（bMSCs）在大鼠脑内的迁移和分化情况。方法体外分离培养大鼠四肢骨的bMSCs，原代培养8～10d后, 同时用含增强型绿色荧光蛋白（EGFP）的反转录病毒上清转导bMSCs并进行抗性筛选，7～10d可得到稳定表达EGFP的bMSCs，然后将用EGFP标记的bMSCs通过侧脑室和尾静脉两种注射方式分别注入纹状体损伤的大鼠体内，进而观察bMSCs向脑内迁移和分化的情况。结果在移植后的大鼠脑内均观察到两种方式植入大鼠体内的bMSCs，这些EGFP阳性的细胞呈现出一些神经元和神经胶质细胞的形态并能表达一些神经元和神经胶质细胞的特异性标志物。结论骨髓基质细胞中确实含有能够向神经元方向分化的干细胞。

关键词: 绿色荧光蛋白, 骨髓基质细胞, 迁移, 分化

Abstract: Objective To investigate the potential of bone marrow stromal cells (bMSCs) to migrate and differentiate into the neural cells in vivo. Methods bMSCs were isolated by a density gradient (lymphocytes separation) from rats' limbs. After culturing for 8 to 10 days in primary culture, the bMSCs were transduced by retrovirus supernatants of EGFP from PT67 cells. The transduced bMSCs were then selected by G418. After being selected for 7 to 10 days, bMSCs could stably express EGFP. Then these bMSCs marked by EGFP were transplanted into the striatum of injured rats by tail veins or lateral ventricle. Results bMSCs were observed in the brain and capable of the morphology of neural cells and expressing some neural antigens. Conclusion Our findings suggest the migration and neural differentiation potential of bone mensenchymal stem cells and the potential use of bMSCs for a cell therapy in CNS diseases.

Key words: green fluorescent protein, bone mesenchymal stem cells, migration, differentiation

段春礼姬曼孙晓红赵春礼杨慧. 大鼠骨髓基质细胞在脑内的迁移和分化[J]. 基础医学与临床, 2008, 28(5): 432-435.

Chun-li DUAN; Man JI; Xiao-hong SUN; Chun-li ZHAO; Hui YANG. Migration and neural differentiation potential of rat bone marrow stromal cells[J]. Basic & Clinical Medicine, 2008, 28(5): 432-435.

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