基础医学与临床 ›› 2008, Vol. 28 ›› Issue (2): 196-197.

• 研究短文 • 上一篇    下一篇

梗阻性黄疸大鼠小肠组织IgA及白介素-6mRNA的表达

姜旭 李哲浩 翟晓芳 崔凤奎   

  1. 吉林省延边大学附属医院 吉林省延边大学附属医院 吉林省延边大学附属医院 吉林省延吉市局子街119 吉林省延边大学附属医院 吉林省延吉市局子街119
  • 收稿日期:2007-01-10 修回日期:2007-05-08 出版日期:2008-02-25 发布日期:2008-02-25
  • 通讯作者: 李哲浩

Expression of the IgA and interleukin-6mRNA on intestinal tissues of Rats with obstructive jaundice

Xu JIANG, Zhe-hao LI, Xiao-fang ZHAI, Feng-kui CUI   

  • Received:2007-01-10 Revised:2007-05-08 Online:2008-02-25 Published:2008-02-25
  • Contact: Zhe-hao LI,

摘要: 目的 探讨梗阻性黄疸时小肠组织免疫球蛋白(Ig)A及白介素(IL)-6mRNA的表达及其意义。方法 健康Wistar雄性大鼠48只,分为假手术组及梗阻性黄疸组,取小肠组织以原位杂交法测IL-6mRNA+细胞数,以免疫组化法测定肠粘膜固有层内IgA+细胞,测定门静脉血内毒素含量。结果 梗阻性黄疸4、7、14d组肠粘膜固有层内IgA+细胞及IL-6mRNA+细胞均较假手术组减少(P<0.01),梗阻性黄疸4、7、14d组间相比,IgA+细胞数及IL-6mRNA+细胞数随胆道梗阻时间延长而减少(P<0.05)。门静脉血内毒素含量与肠粘膜固有层内IgA+细胞呈负相关(r=-0.906 P<0.01);和IL-6mRNA+细胞呈负相关(r=-0.891 P<0.01)。结论梗阻性黄疸时小肠粘膜固有层内IgA及IL-6mRNA的表达减少,是削弱肠粘膜屏障功能的原因之一,是导致肠源性内毒素血症的一个重要原因。

关键词: 黄疸, IgA, 白介素-6, 内毒素, 肠粘膜

Abstract: Objective To investigate the significance of altered intestinal immunoglobulin(Ig)A and interleukin(IL)-6mRNA expression in obstructive jaundice. Methods Forty-eight mail Wistar Rats were divided randomly into shame-operation(SO) group and obstructive jaundice(OJ) group. In situ hybridization and immunohistochemistry were used to detect the expression of IL-6mRNA, IgA in intestinal tissues; and the level of endotoxin in portal vein were detected. Results: The quantity of IgA+, IL-6mRNA+ cells in OJ group were lower than the SO group (P<0.01). And in OJ group the quantity of IgA+, IL-6mRNA+ cells were lessoned with the length of the day (P<0.05). The correlation between quantity of IgA+ cells and the endotoxin level in portal vein was negative (r=-0.906, P<0.01). The correlation between the quantity of IL-6mRNA+ cells and the endotoxin level in portal vein was negative(r=-0.891, P<0.01). Conclusion lower-regulation of IgA and IL-6mRNA expression in the intestinal tissues of rats with obstructive jaundice could impair the function of barrier of intestinal mucosa and it is another reason of induced enterotoxemia.

Key words: jaundice, IgA, interleukin-6, endotoxin, intestinal mucosa