吉林地区大肠埃希菌和肺炎克雷伯菌ESBLs的基因分型

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (2): 164-166.

吉林地区大肠埃希菌和肺炎克雷伯菌ESBLs的基因分型

李坚王艾琳张晓梅宿立娟

北华大学医学院北华大学医学院华北大学附属医院吉林化工二院

收稿日期:2007-04-04 修回日期:2007-05-14 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 李坚

Genotyping of Escherichia coli and Klebsieua pneumoniae producing Extended Spectrum β-lactamases in jilin area of china

Jian LI, Ai-lin WANG, Xiao-mei ZHANG, Li-juan SU

Medical College of Beihua University

Received:2007-04-04 Revised:2007-05-14 Online:2008-02-25 Published:2008-02-25
Contact: Jian LI,

摘要/Abstract

摘要： 目的对产ESBLs大肠埃希菌和肺炎克雷伯菌携带的ESBLs耐药基因进行分型，为临床合理应用抗生素提供理论依据。方法收集住院患者标本中分离出的51株大肠埃希菌和32株肺炎克雷伯菌，经聚合酶链反应(PCR)对上述菌株所携带的ESBLs耐药基因进行分型。结果产ESBLs大肠埃希菌中耐药质粒编码TEM型、SHV型和非TEM非SHV型超广谱β-内酰胺酶基因的百分率依次为80.4%, 7.8%和11.8%;而在肺炎克雷伯菌中的百分率依次为78.1%, 71.9%和25.0%.多数产ESBLs肺炎克雷伯菌同时产生一种以上的β-内酰胺酶。结论获得了吉林地区大肠埃希菌和肺炎克雷伯菌ESBLs的不同基因型，ESBLs基因型具有地区性差异。

Abstract: Objective To study the genotype of strains producing ESBLs among Escherichia coil and Klebsieua pneumoniae to provide references for the clinical application of　drugs. Methods PCR was used to determine the genotype of the ESBLs .Results The percentage of TEM type, SHV type and NO-TEM-NO-SHV type in Escheroichia coil was 80.4%, 7.8% and 11.8%，in Klebsieua pneumoniae was 78.1%, 71.9% and 25.0%. Most of Klebsieua pneumoniae produced more than two Extended Spectrum β-lactamases 。Conclusions the differences in genotype has the regional characteristics.

李坚王艾琳张晓梅宿立娟. 吉林地区大肠埃希菌和肺炎克雷伯菌ESBLs的基因分型[J]. 基础医学与临床, 2008, 28(2): 164-166.

Jian LI; Ai-lin WANG; Xiao-mei ZHANG; Li-juan SU. Genotyping of Escherichia coli and Klebsieua pneumoniae producing Extended Spectrum β-lactamases in jilin area of china[J]. Basic & Clinical Medicine, 2008, 28(2): 164-166.