CYP2J3基因经静脉转染大鼠的组织分布

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (12): 1251-1254.

CYP2J3基因经静脉转染大鼠的组织分布

常静芦玲巧王红霞王晶马立权郑少鹏张立克

首都医科大学病理生理学教研室首都医科大学首都医科大学首都医科大学首都医科大学病理生理学教研室首都医科大学病理生理学教研室

收稿日期:2008-05-04 修回日期:2008-07-21 出版日期:2008-12-25 发布日期:2008-12-25
通讯作者: 张立克

Distribution of CYP2J3 in Gene Transfection Rats by vena dorsalis penis

Jing CHANG, Ling-qiao LU, Hong-xia WANG, Jing WANG, Li-quan MA, Shao-peng ZHENG, Li-ke ZHANG

Department of Pathophysiology, Capital University of Medical Sciences Department of Pathophysiology, Capital University of Medical Sciences

Received:2008-05-04 Revised:2008-07-21 Online:2008-12-25 Published:2008-12-25
Contact: Li-ke ZHANG

摘要/Abstract

摘要： 目的观察CYP2J3基因转染后大鼠心、肝、肺、肾及胸主动脉CYP2J3基因表达和11，12-EET的含量。方法经大鼠阴茎背静脉快速注射质粒复制大鼠转基因模型。将36只雄性 Wistar 大鼠（280~300g）随机分为空白对照组、pcDNA3.1质粒和pcDNA3.1- CYP2J3重组质粒注射组，分别于14和28d取材。用半定量RT-PCR法检测CYP2J3 mRNA表达，高效液相色谱法测定11，12-EET含量。结果 pcDNA3.1-CYP2J3重组质粒组28d时各组织CYP2J3基因表达强于对照组和pcDNA3.1质粒组；各组织11，12－EET 含量增高。（P < 0.05）结论以pcDNA3.1质粒作为非病毒性载体，可增强目的基因CYP2J3在心、肺、肝、肾及胸主动脉的表达。

关键词: CYP450, CYP2J3, 质粒, 基因转染, EETs

Abstract: Objective To detect CYP2J3 gene expression and contents of 11，12-EET in heart, liver, lung, kidney and aorta thoracalis after CYP2J3 gene transfection . Methods The rat transgenic model was built by injecting plasmid through vena dorsalis penis. The animals were divided into control group、 pcDNA3.1 transgenic group and pcDNA3.1- CYP2J3 transgenic group. The expression of CYP2J3 mRNA was detected by RT-PCR method and content of 11，12-EET was examined by the HPLC in heart, liver, lung, kidney and aorta thoracalis at 14 days and 28 days after injection. Results At 28 days after injection, the expression of CYP2J3 mRNA and the content of 11，12-EET were significantly increased than that of control and pcDNA3.1 transgenic group ( P<0.05) . Conclusions The method of injecting plasmid can increase the expression of CYP2J3 in heart, liver, lung, kidney and aorta thoracalis.

Key words: CYP450, CYP2J3, plasmid, gene transfection, EETs

中图分类号:

R541.4

常静芦玲巧王红霞王晶马立权郑少鹏张立克. CYP2J3基因经静脉转染大鼠的组织分布[J]. 基础医学与临床, 2008, 28(12): 1251-1254.

Jing CHANG; Ling-qiao LU; Hong-xia WANG; Jing WANG; Li-quan MA; Shao-peng ZHENG; Li-ke ZHANG. Distribution of CYP2J3 in Gene Transfection Rats by vena dorsalis penis[J]. Basic & Clinical Medicine, 2008, 28(12): 1251-1254.

[1]	侯睿金鑫李和. 人端粒酶反转录酶启动子调控的肿瘤特异性载体的构建及表达[J]. 基础医学与临床, 2013, 33(2): 189-193.
[2]	谌琴何冬梅. Pre-miR-15a增强Raji细胞对阿糖胞苷的敏感性[J]. 基础医学与临床, 2010, 30(9): 991-992.
[3]	朱财林李南林王廷. 转染NDRG2基因抑制乳腺癌细胞增殖[J]. 基础医学与临床, 2010, 30(1): 37-41.
[4]	宋向凤田中伟付丹丹毕新岭. 外源性FHIT 基因对人皮肤癌细胞株A431细胞增殖与凋亡的影响[J]. 基础医学与临床, 2009, 29(4): 405-408.
[5]	廖长秀汪晖平洁吴勇. 基因芯片技术研究乙醇对肝星状细胞增殖及其CYP450亚型表达的影响[J]. 基础医学与临床, 2008, 28(9): 988-989.
[6]	龙香娥龚朝辉. 哺乳动物中介导RNA干扰的载体研究进展[J]. 基础医学与临床, 2008, 28(9): 1004-1006.
[7]	陈巾杰浦晓东. TIMP-3基因瞬时表达对兔血管平滑肌细胞增殖、迁移及凋亡的影响[J]. 基础医学与临床, 2008, 28(7): 756-759.