基础医学与临床 ›› 2008, Vol. 28 ›› Issue (12): 1248-1250.

• 研究论文 • 上一篇    下一篇

Anchor Attachment蛋白在结直肠癌中表达增强

左富义 李世拥 于波(北京) 安萍 杨梅   

  1. 北京军区总医院全军普通外科中心 北京军区总医院全军普通外科中心 北京军区总医院全军普通外科中心 北京军区总医院全军普通外科中心
  • 收稿日期:2007-06-11 修回日期:2008-01-17 出版日期:2008-12-25 发布日期:2008-12-25
  • 通讯作者: 左富义

Anchor Attachment Protein Over-expressed in Colorectal Carcinoma

Fu-yi ZUO, Shi-yong LI, Bo YU, Ping AN, Mei YANG   

  1. Military General Surgery Center, Beijing Military General Hospital
  • Received:2007-06-11 Revised:2008-01-17 Online:2008-12-25 Published:2008-12-25
  • Contact: Fu-yi ZUO,

摘要: 目的 探讨Anchor Attachment蛋白(AAP)的表达与结直肠癌浸润、转移的关系及临床意义。方法 免疫组织化学法检测AAP的表达,分析AAP表达水平与肿瘤临床病理特征之间的关系;并用酶联免疫吸附(ELISA)法检测血清中AAP表达水平。结果 结直肠正常肠黏膜、癌原发灶、淋巴结和肝转移灶中AAP的表达阳性率分别为20.5%、53.0%、69.8%和80.0%;癌原发灶、转移淋巴结和肝转移灶中AAP表达阳性率均显著高于正常肠黏膜组织(P<0.01),转移淋巴结和肝转移灶中AAP阳性率高于癌原发灶(P<0.05);淋巴结转移患者和肝转移患者的原发灶AAP阳性率显著高于无转移者(P<0.01);Dukes分期A、B、C、D期患者AAP阳性率逐渐增高,各分期之间差异显著(P<0.01)。83例结直肠癌患者血清AAP水平为(6.3±2.8)μg/L,显著高于30例健康志愿者的(2.2±0.9)μg/L,(t=6.976,P<0.01);Dukes分期A、B期患者血清AAP水平为(5.2±2.6)μg/L,显著低于C、D期患者的(7.1±2.9)μg/L(P<0.05)。结论 检测外周静脉血AAP水平对预测和判断结直肠癌病情和肝转移有重要意义。

Abstract: Objective To investigate the expression of anchor attachment protein (AAP) in colorectal carcinoma tissues and serum levels of AAP in colorectal carcinoma patients and to study the relationship between AAP expression and lymph node and liver metastases. Methods Immunohistochemistry was used to detect AAP expression, and enzyme linked immunosorbent assay was used to test AAP levels in peripheral vein blood in 83 patients with colorectal carcinoma. Results The expression rates in normal colorectal mucosa, primary cancer, lymph nodes and liver metastases were 20.5%、53.0%、69.8% and 80.0%, respectively. The positive rates of AAP in primary cancer, lymph nodes and liver metastases were higher than that in normal mucosa (P<0.01). The positive rates of AAP in lymph nodes and liver metastases were higher than that in primary cancer (P<0.05). The AAP expression in primary cancer with lymph node and liver metastases were higher than that without metastases (P<0.01). The mean serum level of AAP in 83 patients was (6.3±2.8)μg/L, which was obviously higher than that in control of 30 healthy volunteers (P<0.01). The serum level of AAP in patients with Dukes C and D was (7.1±2.9)μg/L, which was markedly higher than that with Dukes A and B (5.2±2.6)μg/L (P<0.05). Conclusions Monitoring the serum level in patients with colorectal carcinoma is important in prediction and diagnosis of pathogenetic condition and liver metastasis.