感染性休克大鼠内源性Apelin及其受体系统表达下调

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (11): 1183-1186.

感染性休克大鼠内源性Apelin及其受体系统表达下调

薛林潘春水滕旭赵春玉蔡嫣唐朝枢齐永芬

北京大学第一医院心内科北京大学第一医院心内科北京大学医学部生理与病理生理学系北京大学第一医院心内科北京大学医学部生理与病理生理学系

收稿日期:2008-01-08 修回日期:2008-02-25 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 薛林

Down regulation on endogenous apelin and its receptor expression in the rats with septic shock

Lin XUE, Chun-shui PAN, Xu TENG, Chun-yu ZHAO, Yan CAI, Chao-shu TANG, Yong-fen QIN

Dept of cardiology, Peking University the First Hospital, Beijing 100034 Dept of cardiology, Peking University the First Hospital, Beijing 100034 Dept of Phhysiology and Pathophysiology, Peking University Health Science Center Dept of cardiology, Peking University the First Hospital, Beijing 100034 Dept of Phhysiology and Pathophysiology, Peking University Health Science Center

Received:2008-01-08 Revised:2008-02-25 Online:2008-11-25 Published:2008-11-25
Contact: Lin XUE,

摘要/Abstract

摘要： 目的观察内源性Apelin及其受体(血管紧张素受体AT1 相关蛋白,APJ)在盲肠结扎和穿孔所致感染性休克大鼠的变化。方法：20只雄性Wistar 大鼠，体重220～250g, 随机分为假手术对照组和晚期休克组。酶联免疫法测定血浆和心肌中Apelin含量，RT-PCR法测定心肌组织Apelin、APJ mRNA水平，Western blot方法测定心肌组织中APJ蛋白水平。结果：与假手术组比较，休克组大鼠+LVdP/dtmax 和-LV dP/dt max明显降低；左心室舒张末期压(LVEDP)增高，平均动脉压降低，心率增快；有严重低血糖症和高乳酸血症。血浆和心肌Apelin含量明显降低（P<0.01）,心肌组织Apelin、Apelin受体APJ mRNA 水平显著下降(P<0.01)，心肌组织APJ蛋白水平明显降低(P<0.01)。结论：Apelin及其受体系统下调在感染性休克发生发展中可能有重要作用。

Abstract: Objective To observe the variation of endogenous Apelin with its APJ receptor on the model of rats with septic shock are caused by cecal ligation and puncture (CLP). Methods: Twenty male Wistar rats weighting 200~250g randomized into two groups; control group, severe septic shock group. Enzyme immunoassay (EIA) was used to detect levels of Apelin in plasma and myocardia. RT-PCR was used to analysis the relative amount of mRNA of Apelin and APJ in the myocardia. Western blot was used to determine the protein levels of myocardial APJ. Result: Compared with sham, rats with sepsis showed inhibited cardiac function (+LVdP/dtmax and -LVdP/dtmax decreased, and LVEDP elevated). Rats with septic shock showed significant hypoglycemia and hyperlacticaemia, lower levels of Apelin in plasma and myocardia, decreased mRNA relative amount of Apelin and APJ in the myocardia, and lower myocardial APJ protein level. Conclusion: Apelin and its receptor might play an important role in septic shock.

薛林潘春水滕旭赵春玉蔡嫣唐朝枢齐永芬. 感染性休克大鼠内源性Apelin及其受体系统表达下调[J]. 基础医学与临床, 2008, 28(11): 1183-1186.

Lin XUE; Chun-shui PAN; Xu TENG; Chun-yu ZHAO; Yan CAI; Chao-shu TANG; Yong-fen QIN. Down regulation on endogenous apelin and its receptor expression in the rats with septic shock[J]. Basic & Clinical Medicine, 2008, 28(11): 1183-1186.