PARP抑制剂5-AIQ对小鼠结肠癌CT26细胞PARP/NF-κB复合物和NF-κB活性的影响

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (11): 1156-1159.

PARP抑制剂5-AIQ对小鼠结肠癌CT26细胞PARP/NF-κB复合物和NF-κB活性的影响

蔡莉王娅兰林晓

重庆医科大学病理教研室重庆医科大学病理教研室重庆医科大学病理教研室

收稿日期:2007-10-15 修回日期:2008-03-10 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 王娅兰

Effect of PARP inhibitor 5-AIQ on PARP/NF-κB complex and NF-κB activity in murine colon carcinomaCT26 cells

Li CAI, Ya-lan WANG, Xiao LIN

Dept. of Pathology, Chongqing Medical University Dept. of Pathology, Chongqing Medical University Dept. of Pathology, Chongqing Medical University

Received:2007-10-15 Revised:2008-03-10 Online:2008-11-25 Published:2008-11-25
Contact: Ya-lan WANG,

摘要/Abstract

摘要： 目的探讨PARP抑制剂5-AIQ对小鼠结肠癌CT26细胞核内PARP抑制后，对PARP/NF-κB p65复合物形成和NF-κB活性的影响。方法 Western blot检测结肠癌CT26细胞核内PARP与NF-κBp65的表达，免疫共沉淀法分析CT26细胞核内PARP/NF-κB p65复合物形成，电泳迁移率改变分析法检测CT26细胞核转录因子NF-κB的结合活性。结果 5-AIQ处理组与5-AIQ未处理组比较，5-AIQ处理组小鼠结肠癌CT26细胞核内PARP与NF-κBp65的表达水平均明显减弱（P<0.05），PARP/NF-κBp65复合物形成明显减少（P<0.05），NF-κB的活性显著降低（P<0.05）。结论 5-AIQ可通过抑制PARP的表达，减少PARP/NF-κBp65复合物的形成，进而使NF-κB活性降低。

Abstract: Objective: The aim is to investigate the effect of PARP inhibitor 5-AIQ on PARP/NF-κBp65 complex and the NF-κB activity in murine colon carcinoma CT26 cell lines．Methods: The murine colon carcinoma CT26 cell lines were treated with 5-AIQ in vitro．The expressions of PARP and NF-κBp65 were analyzed by Western Blot. The PARP/NF-κBp65 complex was analyzed by co-immunoprecipitation. The NF-κB binding activity was detected by electrophoretic mobility shift assay (EMSA). Results: Compared with 5-AIQ-untreated group, the expressions of PARP and NF-κBp65 were markedly decreased in 5-AIQ-treated colon carcinoma CT26 cell groups (P<0.05). The PARP/NF-κBp65 complex was decreased in 5-AIQ-treated colon carcinoma CT26 cell group (P<0.05).The NF-κB DNA binding activity was also reduced in 5-AIQ-treated colon carcinoma CT26 cell groups(P<0.05). Conclusions：These results suggest that 5-AIQ can inhibit PARP expression in murine colon carcinoma CT26 cells, and further inhibit the formation of PARP/NF-κBp65 complex and NF-κB activity.

蔡莉王娅兰林晓. PARP抑制剂5-AIQ对小鼠结肠癌CT26细胞PARP/NF-κB复合物和NF-κB活性的影响[J]. 基础医学与临床, 2008, 28(11): 1156-1159.

Li CAI; Ya-lan WANG; Xiao LIN. Effect of PARP inhibitor 5-AIQ on PARP/NF-κB complex and NF-κB activity in murine colon carcinomaCT26 cells[J]. Basic & Clinical Medicine, 2008, 28(11): 1156-1159.