Abstract: Objective To investigate the effect and mechanism of FRNK on proliferation of Hepatic Stellate Cells in vitro. Methods After FN stimulated HSCs, FRNK plasmid mediated by cationic liposome was transfected into HSCs. The proliferation of HSCs was evaluated by improved MTT assay. And the levels of FRNK, FAK, p-FAK (Tyr397), ERK1 and p-ERK in HSCs were assayed by Western blot and RT-PCR. Results Compared with nFRNK plasmid group，FRNK inhibited the proliferation of HSCs，and the inhibition rates at 12，24 and 48 h were 20.07 %, 26.16 % and 29.77 %( P<0.01), respectively. Compared with the nFRNK plasmid group, the expression of p-FAK, ERK1 and p-ERK in FRNK plasmid group reduced; On the contrary, compared with the control group, the expression of p-FAK, ERK1 and p-ERK in FN group increased. Conclusions FRNK can inhibit the HSCs proliferation in a time-dependent manner and FAK-ERK signal transduction pathway was perhaps involved in the process.