基础医学与临床 ›› 2008, Vol. 28 ›› Issue (11): 1134-1137.

• 研究论文 • 上一篇    下一篇

口腔鳞癌患者淋巴细胞对人疱疹病毒6型增殖应答受损

王芳 姚堃 周锋 杨婕   

  1. 南京医科大学 第一附属医院(江苏省人民医院)临床检验中心 南京医科大学 微生物学与免疫学系 南京医科大学 微生物学与免疫学系 南京医科大学 口腔医学院
  • 收稿日期:2007-09-24 修回日期:2008-04-15 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 姚堃

Impairment of lymphoproliferative response to human herpesvirus-6 in oral squamous cell carcinoma patients

Fang WANG, Kun YAO, Feng ZHOU, Jie YANG   

  1. 1.Dept. Clinical Laboratories, the First Affiliated Hospital, Nanjing Medical University Dept. Immonology and Microbiology,Nanjing Medical University
  • Received:2007-09-24 Revised:2008-04-15 Online:2008-11-25 Published:2008-11-25
  • Contact: Kun YAO,

摘要: 目的 研究口腔鳞癌患者淋巴细胞对人疱疹病毒6型(HHV-6)特异性增殖应答,初步探讨HHV-6在口腔鳞癌发病机制中的作用。方法 间接免疫荧光法(IIF)检测血浆中抗HHV-6 IgG;免疫微磁珠分离CD4+ T及CD8+ T细胞;3H-TdR法检测CD4+T和CD8+T细胞及PBMCs的增殖水平;FACS分析CD4+CD25+ 调节性T细胞(Treg)的比例。 结果 口腔鳞癌组血浆中抗HHV-6 IgG阳性数为8/8,正常对照组为12例(12/20);口腔鳞癌组PBMCs及CD4+ T细胞对HHV-6的增殖水平显著低于HHV-6潜伏感染组与未感染组(P< 0.05);HHV-6潜伏感染组PBMCs及CD4+ T细胞对HHV-6的增殖水平显著低于未感染组(P< 0.05);口腔鳞癌组外周血中CD4+CD25+Treg比例明显高于HHV-6潜伏感染组与未感染组(P<0.05)。 结论 口腔鳞癌患者的HHV-6特异性CD4+ T细胞增殖应答减弱,可能在口腔鳞癌的发生发展中起一定作用。

关键词: 人疱疹病毒6型, 口腔鳞癌, 淋巴细胞增殖, CD4+CD25+调节性T细胞

Abstract: Objective To study the lymphoproliferative responses to HHV-6 (Human Herpesvirus-6, HHV-6)among patients with oral squamous cell carcinoma, and discuss the role of HHV-6 in the pathogenesis of oral squamous cell carcinoma. Methods IgG antibody to HHV-6 in plasma were identified by means of indirect immunofluorescence (IIF). Immuno-magnetic beads were used to prepare CD4+ T cells and CD8+ T cells. The proliferation of CD4+ T cells, CD8+ T cells and PBMCs stimulated with HHV-6 or anti-CD3 antibodies were measured by means of 3H-thymidine uptake. The percentage of CD4+ CD25+ Treg cells(Regulatory T cell,Treg)within the peripheral blood CD4+ T cell compartment was analyzed by flow cytometry. Results Significantly higher proportion of patients with oral squamous cell carcinoma had IgG antibody to HHV-6 (8/8 ) in plasma compared with those in control subjects (12/20 );The proliferative responses of PBMCs and CD4+ T cells from patients withoral squamous cell carcinoma to HHV-6 were significantly decreased compared to those from HHV-6-infected and uninfected healthy individuals(P< 0.05;P< 0.05). The proliferative responses of PBMCs and CD4+ T cells from HHV-6-infected healthy individuals to HHV-6 were significantly decreased compared to those from HHV-6-uninfected healthy individuals(P< 0.05);Patients with oral squamous cell carcinoma had a significantly higher percentage of CD4+ CD25+ Treg cells within the peripheral blood CD4+ T cell compartment compared with HHV-6 infected and uninfected healthy individuals did. Conclusion These results demonstrated that impairment of HHV-6-specific CD4+ T cell immune responses in patients with oral squamous cell carcinoma, therefore, HHV-6 possibly plays a role in the pathogenesis of oral squamous cell carcinoma.

Key words: Human herpesvirus-6, oral squamous cell carcinoma, lymphoproliferation, CD4+ CD25+ Treg cell