基础医学与临床 ›› 2008, Vol. 28 ›› Issue (10): 1025-1029.

• 研究论文 • 上一篇    下一篇

天冬氨酸异羟肟酸对大鼠血压以及血管重塑的影响

赵霞 金红芳 杜淑旭 陈跃 唐朝枢 杜军保   

  1. 北京大学第一医院儿科 北京大学第一医院儿科 北京大学第一医院儿科 北京大学医药卫生分析中心 北京大学第一医院心血管病研究所 北京大学第一医院儿科
  • 收稿日期:2007-12-05 修回日期:2008-01-31 出版日期:2008-10-25 发布日期:2008-10-25
  • 通讯作者: 金红芳

Effect of hydroxamate on blood pressure and vascular remodeling in rats

Xia ZHAO, Hong-fang JIN, Shu-xu DU, Yue CHEN, Chao-shu TANG, Jun-bao DU   

  • Received:2007-12-05 Revised:2008-01-31 Online:2008-10-25 Published:2008-10-25
  • Contact: Hong-fang JIN,

摘要: 目的 观察二氧化硫(SO2)生成酶抑制剂天冬氨酸异羟肟酸(HDX)对大鼠血压以及血管结构的影响。方法 4周龄雄性自发性高血压大鼠(SHR)及WKY大鼠随机分为对照组及HDX组(各8只,共32只)。5周后检测血压、胸主动脉与血浆SO2水平、GOT活性、增值指数(PI)和p-ERK蛋白表达,胸主动脉显微形态结构。结果 HDX干预后,WKY大鼠血压、中膜厚度与内径之比、PCNA、p-ERK蛋白表达均明显升高,而血浆及胸主动脉SO2水平及GOT活性明显降低(P < 0.05 or P < 0.01);SHR胸主动脉与血浆SO2水平、GOT活性明显低于WKY组(P < 0.05 or P < 0.01)。 结论 内源性SO2是维持机体血压及血管结构正常的重要因素之一。

Abstract: Objective To investigate the effect of hydroxamate (HDX, inhibitor of endogenous sulfur dioxide production) on blood pressure and vascular structure in rat. Methods 4-week-old SHRs (spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats were divided into control and HDX-treated group (respectively, n = 8). Five weeks later, the blood pressure, SO2 content and GOT activity in serum and aorta, morphometric parameters, proliferative index (PI) and the expression of p-ERK were determined. Results Compared with WKY rats, the blood pressure, ratio of wall thickness to lumen radius, PI and the expression of p-ERK in aorta were increased, while the SO2 content and GOT activity in serum and aorta were decreased in WKY+HDX groups (P < 0.05 or P < 0.01). The SO2 content and GOT activity in serum and aorta of SHR were lower than WKY rat (P < 0.05 or P < 0.01). Conclusions Endogenous sulfur dioxide might play a pivotal role in maintaining normal pressure and vascular structure.