Apelin-13刺激大鼠血管平滑肌细胞增殖

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (1): 9-12.

Apelin-13刺激大鼠血管平滑肌细胞增殖

李峰李兰芳秦旭平潘伟男朱炳阳廖端芳陈临溪

湖南衡阳南华大学药物药理研究所湖南衡阳南华大学药理教研室湖南衡阳南华大学药物药理研究所湖南衡阳南华大学药物药理研究所湖南衡阳南华大学药物药理研究所湖南衡阳南华大学药物药理研究所湖南衡阳南华大学药物药理研究所

收稿日期:2006-12-04 修回日期:2007-03-27 出版日期:2008-01-25 发布日期:2008-01-25
通讯作者: 陈临溪

Apelin-13 stimulation of vascular smooth muscle cells proliferation in rats

Feng LI, Lan-fang LI, Xu-ping QIN, Wei-nan PAN, Bing-yang ZHU, Duan-fang LIAO, Lin-xi CHEN

Received:2006-12-04 Revised:2007-03-27 Online:2008-01-25 Published:2008-01-25
Contact: Lin-xi CHEN

摘要/Abstract

摘要： 目的研究G蛋白耦联受体APJ的内源性配体多肽apelin-13对大鼠血管平滑肌细胞(VSMCs)增殖的影响及其与ERK1/2信号通路的关系。方法采用噻唑蓝比色法（MTT）、Western blot等方法观察apelin-13对体外培养大鼠VSMCs增殖的影响及细胞周期蛋白表达的变化。结果 apelin-13浓度依赖性促进VSMCs增殖，且cyclin D1表达增加；ERK1/2阻断剂PD98059可明显抑制apelin-13诱导的细胞增殖及pERK1/2、cyclin D1表达。结论 apelin-13能促进大鼠VSMCs增殖，其机制可能与apelin/APJ-ERK1/2－cyclin D1信号通路有关。

Abstract: Objective To observe the effect of G protein-coupled receptor APJ endogenous ligand apelin-13 on the proliferation of vascular smooth muscle cells (VSMCs),and investigate whether the effect is related to apelin/APJ-pERK1/2 -cyclin D1 signal transduction pathway. Methods The cell proliferation were measured by MTT assay and the expression of cyclin D1 and pERK1/2 were determined by Western blot.The rusults showed that apelin-13 can stimulated VSMC proliferation in a concentration-dependent manner. Western blot analysis showed that apelin-13 stimulated the expression of cyclin D1 and pERK1/2. The ERK1/2 inhibitor PD98059 decreased the expression of pERK1/2 induced by apelin-13. Similarly, PD98059 partially diminished the expression of cyclin D1, and inhibited the VSMCs proliferation stimulated by apelin-13. Conclusion Apelin-13 can stimulated the proliferation of VSMC and the effect was possibly mediated by apelin/APJ- pERK1/2-cyclin D1 signal cascades.

李峰李兰芳秦旭平潘伟男朱炳阳廖端芳陈临溪. Apelin-13刺激大鼠血管平滑肌细胞增殖[J]. 基础医学与临床, 2008, 28(1): 9-12.

Feng LI; Lan-fang LI; Xu-ping QIN; Wei-nan PAN; Bing-yang ZHU; Duan-fang LIAO; Lin-xi CHEN. Apelin-13 stimulation of vascular smooth muscle cells proliferation in rats[J]. Basic & Clinical Medicine, 2008, 28(1): 9-12.