Abstract: Objective To investigate the influence of Angiopoietin-1(Ang1) gene modified mesenchymal stem cells on the expression of adhension factors on endothelial cells, to clarify the feasibility of modulating inflammation reaction by gene modification after stem cells transplantation. Methods Genetic engineering rMSCs were constructed by lentivirally-tranduced Ang1 gene, the expressions of ICAM-1 and VCAM-1 at different time point after 20μg/L VEGF stimulating were investigated, the supernatants of gene modified rMSC were collected and then added to HUVEC culture with different concentration of Ang1, the expressions of adhension factors after VEGF stimulated were observed at the same time. Results Genetic engineering rMSCs were successfully constructed, the expressions of ICAM-1 and VCAM-1 mRNA were significantly increased after VEGF stimulated, the maximal value was at 8h, the ICAM-1 and VCAM-1 mRNA expressions were decreased after cultured with Ang1 at different concentration, Ang1 suppressed the VEGF-stimulated protein levels of ICAM-1 and VCAM-1. Conclusion The supernatants of Ang1 gene modified stem cells can suppressed VEGF-stimulated inflammation reaction of HUVEC.