基础医学与临床 ›› 2008, Vol. 28 ›› Issue (1): 22-25.

• 研究论文 • 上一篇    下一篇

微环境对体外间充质干细胞向心肌样细胞分化的作用

张本斯 李庄 杨新文 洪虹 洪永华   

  1. 云南大理学院基础医学院人体解剖学教研室 云南大理学院附属医院胸心外科 云南大理学院基础医学院人体解剖学教研室 云南大理学院基础医学院人体解剖学教研室 云南大理学院基础医学院人体解剖学教研室
  • 收稿日期:2006-12-12 修回日期:2007-03-27 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 张本斯

Role of different microenvironment on differentiation in vitro of mesenchymal stem cells into cardiomyogenic cells

Ben-si ZHANG, Zhuang LI, Xin-wen YANG, Hong HONG, Yong-hua HONG   

  1. Department of Anatomy, Faculty of Basic Medicine, Dali University
  • Received:2006-12-12 Revised:2007-03-27 Online:2008-01-25 Published:2008-01-25
  • Contact: Ben-si ZHANG,

摘要: 目的 研究心肌组织中何种细胞对骨髓间充质干细胞 (MSCs)向心肌样细胞定向分化起决定性作用。方法 分离培养骨髓MSCs、心肌细胞(CM)和内皮细胞(EC)。MSCs经BrdU标记后与CM、EC分别共培养和单独培养,通过形态学、免疫细胞化学和双重免疫细胞化学鉴定。结果 分离培养的MSCs、CM和EC纯度达>95%。标记MSCs与CM共培养后,细胞具有心肌细胞样形态。4w时,有44%的细胞BrdU与肌动蛋白(Sarcomeric Actin)或连接蛋白-43(Connexin-43)双重免疫细胞化学染色阳性。与EC共培养以及单独培养时,MSCs形态变化不明显,无双染阳性细胞出现。结论 CM对骨髓MSCs向心肌样细胞的定向分化具有重要作用。

Abstract: Objective To study which kind of cells in myocardium playing determining role in cardiomyogenic differentiation of marrow derived-mesenchymal stem cells (MSCs). Methods Rat marrow derived-MSCs, cardiomyocytes (CM) and endothelial cells (EC) were isolated and cultured, then MSCs were incubated with the CM or EC or cultured alone after labeled by 5-Bromo-2-deoxyuridine (BrdU) for 72 hours. The cells were identified by morphology, immunocytochemistry and double immunocytochemistry. Results More than 95% of the cells isolated and cultured belonged to MSCs, CM or EC. Incubated with CM, most MSCs labeled by BrdU showed cardiomyocyte-like shape. After 4 weeks of cultivation, positive staining of double immunocytochemistry of BrdU and Sarcomeric Actin or Connexin-43 (CX-43) were observed on 44% cells. However, there was no obvious change in morphology and no positive staining of double immunocytochemistry on MSCs incubated with EC or cultured alone after 1、2、3 and 4 weeks of cultivation. Conclusion MSCs can differentiate into cardiomyogenic cells under cardiomyocyte microenvironment in vitro, and cardiomyocytes may play a crucial role for the differentiation.