Abstract: Objective: To study the modulatory effects of Zn2+ on ATP-activated Currents (IATP) of Xenopus Oocytes. Methods: The two electrode-voltage clamp technique was used to record IATP in healthy Xenopus oocytes by applying Zn2+ in extracellular fluid. Results: We found that IATP (especially the component D1) can be obviously modulated by Zn2+ in a concentration-dependent manner. The amplitude of IATP can be increased by (117.6±8.21)％(n＝11) when modulated by 3×10-5 mol/L Zn2+, which shows a remarkable difference compared with the control value (P<0.01). Zn2+ can cause the concentration-response relationship curve to move towards up-left direction, while does not change the maximal amplitude. Conclusions: The modulation effect of Zn2+ on IATP belongs to allosteric modulation, and may be achieved by changing the affinity of ligand-receptor.