肼苯哒嗪对宫颈癌细胞系APC基因异常甲基化的影响

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (9): 981-984.

肼苯哒嗪对宫颈癌细胞系APC基因异常甲基化的影响

宋银宏张昌菊

湖北宜昌三峡大学医学院湖北省宜昌市三峡大学医学院免疫学科

收稿日期:2006-11-07 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25
通讯作者: 宋银宏

Effect of Hydralazine on the methylation of APC in cervical carcinoma cell lines

Yin-hong Song Chang-ju Zhang

Received:2006-11-07 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25
Contact: Yin-hong Song

摘要/Abstract

摘要： 目的　检测宫颈癌细胞系HeLa、CaSki和SiHa中结肠腺瘤性息肉病基因(APC)启动子区CpG岛甲基化状态；探索肼苯哒嗪(Hyd)能否作为去甲基化药物恢复APC表达。方法　应用甲基化特异性聚合酶链反应(MSP)检测细胞系中APC启动子区CpG岛甲基化情况；Hyd处理后，RT-PCR检测APC mRNA表达。结果　APC启动子区CpG岛在HeLa细胞中被甲基化，在CaSki细胞中半甲基化，在SiHa细胞中无甲基化；一定浓度Hyd处理HeLa、CaSki细胞后，检测到APC mRNA表达。结论　HeLa、CaSki细胞系APC表达沉默与APC启动子区甲基化有关；Hyd能作为去甲基化药物诱导APC表达。

Abstract: 　Objective　To detect the 5′CpG island methylation of APC( adenomatous polyposis coli) gene promoter region in cervical carcinoma cell lines (HeLa,CaSki,SiHa), and to investigate the possibility of Hydralazine in restoring the expression of APC gene through demethylation. Methods　The CpG island methylation status of APC gene promoter region of the cervical carcinoma cell lines were analyzed using methylated specific polymerase chain reaction (MSP). The mRNA expression profile of APC gene was analyzed by using RT-PCR after Hydralazine treatment. Results　 The CpG island of APC gene was methylated in HeLa and hemimethylated in CaSki but not in SiHa. The mRNA expression of APC gene can be detected after a certain concentration Hydralazine treatment. Conclusion The APC gene non-expression in cervical carcinoma is associated with CpG island methylation in APC gene promoter region. Hydralazine, served as a demethylating agent, enables to restore the expression of APC gene.

宋银宏张昌菊. 肼苯哒嗪对宫颈癌细胞系APC基因异常甲基化的影响[J]. 基础医学与临床, 2007, 27(9): 981-984.

Yin-hong Song Chang-ju Zhang. Effect of Hydralazine on the methylation of APC in cervical carcinoma cell lines[J]. Basic & Clinical Medicine, 2007, 27(9): 981-984.