多房棘球绦虫重组BCG-EmⅡ/3疫苗促进小鼠脾细胞因子的分泌

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (9): 966-969.

多房棘球绦虫重组BCG-EmⅡ/3疫苗促进小鼠脾细胞因子的分泌

李文桂王鸿朱佑明

重庆医科大学附属第一医院传染病寄生虫病研究所重庆医科大学附属第一医院传染病寄生虫病研究所重庆医科大学附属第一医院传染病寄生虫病研究所

收稿日期:2006-09-12 修回日期:2006-12-05 出版日期:2007-09-25 发布日期:2007-09-25
通讯作者: 李文桂

Cytokines secretion in splenocytes of mice was promoted by recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis

Wen-gui Li Hong Wang You-ming Zhu

Received:2006-09-12 Revised:2006-12-05 Online:2007-09-25 Published:2007-09-25
Contact: Wen-gui Li

摘要/Abstract

摘要： 目的动态观察多房棘球绦虫重组BCG-EmⅡ/3疫苗免疫小鼠后脾细胞因子的变化。方法采用鼻腔内接种疫苗免疫BALB/C鼠，在免疫后0、2、4、6、8和10周各剖杀4只小鼠取脾，分离脾细胞，用EmAg或ConA刺激培养，收集脾细胞培养上清液，检测脾细胞培养上清液中的IL-2、IFN-γ、TNF-α和IL-4水平，同时设有PBS对照。结果疫苗接种组中IL-2、IFN-γ、TNF-α和IL-4分别在免疫后2～6、2～6、2～10和8～10周升高，分别在免疫后4、4、8和10周达最高水平。结论多房棘球绦虫重组BCG-EmⅡ/3疫苗在免疫早期（2～8周）即可诱导小鼠产生一个TH1型保护性免疫反应。

Abstract: Objective To dynamically observe changes of cytokines of splnocytes in mice by immunization with recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis(Em).Methods BALB/C mice were intranasally vaccinated by the vaccine and killed to get spleen on 0、2、4、6、8 and 10w of immunization respectively, splenocytes were separated to culture by stimulation with EmAg or ConA，their supernatants were gathered to measure level of IL-2,IFN-γ,TNF-α and IL-4 by ELISA Kits. PBS was served as control. Results In the groups of immunization, levels of IL-2,IFN-γ,TNF-α and IL-4 increased obviously on 2～6、2～6、2～10 and 8～10w respectively, reached the highest level on 4、4、8 and 10w respectively. Conclusion TH1 response was induced in mice by rBCG-EmII/3 vaccine on early immunization（2～8w）.

李文桂王鸿朱佑明. 多房棘球绦虫重组BCG-EmⅡ/3疫苗促进小鼠脾细胞因子的分泌[J]. 基础医学与临床, 2007, 27(9): 966-969.

Wen-gui Li Hong Wang You-ming Zhu. Cytokines secretion in splenocytes of mice was promoted by recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis[J]. Basic & Clinical Medicine, 2007, 27(9): 966-969.