人脐血造血干细胞体外扩增方法初探

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (9): 1048-1052.

人脐血造血干细胞体外扩增方法初探

曲鑫建李惠侠丰美福孙世铎

西北农林科技大学 .西北农林科技大学动物科技学院中国科学院动物研究所生物膜与膜生物国家重点工程实验室西北农林科技大学

收稿日期:2006-09-06 修回日期:2006-12-13 出版日期:2007-09-25 发布日期:2007-09-25
通讯作者: 曲鑫建

Investigation of the effectiveness of ex vivo expansion of human umbilical cord hematopoietic stem cells

Xin-jian Qu Hui-xia Li Mei-fu Feng Shi-duo Sun

Animal Science Department of Northwest Sci-Tech University of Agriculture and Forestry, State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zzoology Chinese Academy of sciences .Youjiang Medical Ccollege for Nationalitie

Received:2006-09-06 Revised:2006-12-13 Online:2007-09-25 Published:2007-09-25
Contact: Xin-jian Qu

摘要/Abstract

摘要： 目的建立人造血干细胞(HSC)体外大量扩增的最佳培养模型。方法采用模拟骨髓微环境的成骨细胞共培养法、条件培养基及模拟微重力效应三维培养等方法进行人脐带血来源HSC体外扩增。结果在用含15%的条件培养基与成骨细胞共培养条件下，HSC的扩增倍数最高，在第7天和第14天扩增倍数分别为6.46和14.82倍。在此条件下进行集落形成实验对扩增后造血干细胞多能性的功能检测发现,粒细胞-巨噬细胞集落形成单位(CFU-GM)及红系集落形成单位(BFU-E)数目也最多。结论以成骨细胞作为饲养层细胞，添加15%的条件培养基不仅能使HSC在体外大量增殖，并且具有维持HSC自我更新和多向分化潜能。

Abstract: Objective To investigate the effectiveness of in vitro expansion of human umbilical cord hematopoietic stem cells (HSCs) and to optimize the culture conditions. Methods HSCs were cocultured with osteoblasts in the three-direction (3D) culture system or condition medium (CM) to study the expansion effect on HSCs. Results In the conditions of cocultured with osteoblasts and added with 15%CM produced an expansion of the HSCs at a higher level , the HSCs were evaluated that expansion 6.46-fold after 7th days incubation while 14.82-fold after 14th days incubation. At the same conditions, the number of colony forming units of granulocyte and macrophage (CFU-GM) and erthroid burst-forming unit (CFU-E) were also significant higher than that of other groups. Conclusions The HSCs were cocultured with osteoblast in 15% CM medium not only to achieve admirable results for in vitro expansion HSCs but also sustain the self-renew capacity.

曲鑫建李惠侠丰美福孙世铎. 人脐血造血干细胞体外扩增方法初探[J]. 基础医学与临床, 2007, 27(9): 1048-1052.

Xin-jian Qu Hui-xia Li Mei-fu Feng Shi-duo Sun. Investigation of the effectiveness of ex vivo expansion of human umbilical cord hematopoietic stem cells[J]. Basic & Clinical Medicine, 2007, 27(9): 1048-1052.