基础医学与临床 ›› 2007, Vol. 27 ›› Issue (9): 1043-1047.

• 技术与方法 • 上一篇    下一篇

使用Y染色体特异性探针追踪检测大鼠脑内移植细胞

李娜' 赵焕英 杨慧   

  1. 首都医科大学北京市神经科学研究所
  • 收稿日期:2006-12-06 修回日期:2006-12-27 出版日期:2007-09-25 发布日期:2007-09-25
  • 通讯作者: 李娜'

Detection of the transplanted cells in rat brains by ISH using probes on Y chromosomes

Na Li Huan-ying Zhao Hui Yang   

  • Received:2006-12-06 Revised:2006-12-27 Online:2007-09-25 Published:2007-09-25
  • Contact: Na Li

摘要: 目的 探讨准确高效追踪检测脑内移植来源细胞的方法。方法 克隆雄性大鼠Y染色体SRY基因的一个片段,并且将该片段标记成DNA探针,对大鼠的脑切片进行原位杂交。结果 使用大鼠Y染色体SRY基因片段标记的探针后,可以特异性检测雌性大鼠脑内来源于雄性大鼠的移植细胞。结论 使用原位杂交方法可以有效、准确地追踪检测移植细胞。

Abstract: Objective Developing a method to detect the transplanted cells in rat brains. Methods A SRY gene probe on rat Y chromosomes was cloned and labeled. The probe was used in situ hybridzation (ISH) on female rat brains. Results The experiments showed that the probe of SRY gene could catch the male-derived cells exactly in female brains. Conclution Using ISH of male-derived probe can trace the transplanted cells in female rats.