Abstract: Objective to establish an efficient CD23-expression system. Methods: Human CD23 cDNA was amplified by RT-PCR from lymphocytes isolated from peripheral blood and then inserted into pcDNA3.1A expression vector to construct the pcDNA3.1/CD23 recombinant plasmid. The pcDNA3.1/CD23 plasmids were transfected into HEK293T cells and expressed CD23 protein was detected by flow cytometry and Western blot. Results: The results showed that the sequence of CD23 cDNA amplified was consistent with that previously reported. The highly efficient expression of CD23 in HEK293T cells was achieved after transfection. The high-efficiency transfection (above 80%) and high-level expression were obtained. Conclusion: The human CD23 cDNA was amplified and the high-level expression of CD23 in 293T cells was achieved.