侧脑室注射肾上腺髓质素激活去缓冲神经大鼠最后区神经元

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (5): 505-508.

侧脑室注射肾上腺髓质素激活去缓冲神经大鼠最后区神经元

季淑梅刘俊乐韩笑何瑞荣

燕山大学生物医学工程系燕山大学生物医学工程系燕山大学生物医学工程系

收稿日期:2006-05-08 修回日期:2006-10-17 出版日期:2007-05-25 发布日期:2007-05-25
通讯作者: 季淑梅

Intracerebroventricular administration of adrenomedullin activtes area postrema neurons in sino-aortic denervated rats

Received:2006-05-08 Revised:2006-10-17 Online:2007-05-25 Published:2007-05-25

摘要/Abstract

摘要： 目的观察侧脑室注射肾上腺髓质素（AM）对去缓冲神经大鼠最后区神经元自发放电和Fos蛋白表达的影响。方法应用细胞外记录的电生理学方法，观察雄性SD大鼠神经元自发放电活动；用免疫组化方法检测Fos蛋白的表达。结果侧脑室注射AM (1、3 nmol/kg)诱发最后区出现大量Fos样免疫阳性神经元（Fos-LI），最后区神经元自发放电频率明显增加；降钙素基因相关肽受体拮抗剂CGRP8-37 (30 nmol/kg)可明显减弱AM的效应。结论 AM对最后区神经元有直接兴奋作用，降钙素基因相关肽受体介导这一效应。

Abstract: Objective To examine the effects of intracerebroventricular administration of adrenomedullin (AM) on the expression of Fos and the spontaneous electrical activity of area postrema (AP) neurons in sino-aortic denervated rats. Methods Using immunohistochemistry for Fos and extracellular recording technique, to determine the expression of Fos and the spontaneous electrical activity of AP neurons in male Sprague-Dawley rats, respectly. Results Following Intracerebroventricular administration ofAM (1 nmol/kg, 3 nmol/kg), Fos-like immunoreactive (Fos-LI) 基金项目：河北省教育厅科研项目（2005101） neurons and the discharge rate of AP neurons were markedly increased. Pretreatment with calcitonin gene-related peptide(CGRP) receptor antagonist CGRP8-37 (30 nmol/kg) significantly reduced the effects of AM (3 nmol/kg). Conclusions AM may activate the neurons in AP via CGRP receptors.

季淑梅刘俊乐韩笑何瑞荣. 侧脑室注射肾上腺髓质素激活去缓冲神经大鼠最后区神经元[J]. 基础医学与临床, 2007, 27(5): 505-508.

. Intracerebroventricular administration of adrenomedullin activtes area postrema neurons in sino-aortic denervated rats[J]. Basic & Clinical Medicine, 2007, 27(5): 505-508.