Abstract: Objective: To observe the effect of Vascular Endothelial Growth Factor(VEGF) expression in hepatoma cells affected by TGF-?1 and hopxia . Methods: HepG2 cells were cultured? invitro, and treated with different doses of TGF-?1 and Cobalt chloride hexahydrate(CoCl2), or treated with TGF-?1 and CoCl2. The changes of VEGF protein and mRNA were detected by immunohistochemistry and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Immunohistochemistry and RT-PCR showed that the expression of VEGF protein and mRNA were significantly higher in TGF-β1 groups or CoCl2 groups than that of control group(p<0.01), they increased the expression of VEGF in concentrate-dependently manner. The addition of TGF-β1 with CoCl2 results in significantly more VEGF expression than either stimulation alone(p<0.05). Conclusion: TGF-?1 can increase the expression of VEGFmRNA in Hepatoma cells；TGF-?1 can coordinate with hypoxia induced by CoCl2 to increase the expression of VEGFmRNA.