低氧促进hMSCs 向多巴胺能神经元分化

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (4): 377-381.

低氧促进hMSCs 向多巴胺能神经元分化

赵彤李海生（天津）张翠萍靳冰（北京）丁爱石朱玲玲范明

军事医学科学院基础医学研究所北京武警医学院生理教研室军事医学科学院基础医学研究所北京军事医学科学院基础医学研究所军事医学科学院基础医学研究所军事医学科学院基础医学研究所

收稿日期:2006-03-20 修回日期:2006-07-21 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 赵彤

Hypoxia enhanced the differentiation of human bone marrow-derived mesenchymal stem cells into dopaminergic neurons

Received:2006-03-20 Revised:2006-07-21 Online:2007-04-25 Published:2007-04-25

摘要/Abstract

摘要： 目的观察低氧对体外培养的人骨髓间充质干细胞（hMSCs）向多巴胺能神经元分化的影响。方法采用细胞免疫化学法、流式细胞分析和高效液相色谱-电化学法，检测多巴胺能神经元的数量以及诱导分化后培养基中多巴胺的含量。结果低氧处理后，低氧分化组酪氨酸羟化酶（TH）阳性神经元数量明显增多，为常氧分化组的3倍（P<0.01），并且分化后的神经细胞合成的多巴胺（DA）及其代谢产物高香草酸（HVA）含量也高于常氧分化组（P<0.05）。结论低氧可促进hMSCs向多巴胺能神经元方向分化，这为hMSCs临床应用于治疗帕金森病提供了新的思路。

Abstract: Objective The aim of the study is to investigate the effect of hypoxia on the differentiation of human bone marrow-derived mesenchymal stem cell (hMSCs) into the dopaminerigic neurons. Methods The numbers of differentiated cells were tested by the methods of immunocytochemistry and flow cytometry, and the content of dopamine and its metabolits are measured by HPLC-ECD. Result The number of TH positive cells differentiated from BME-induced hMSCs under hypoxic condition increases about 3 times as compared with BME-induced hMSCs under normoxic condition. Furthermore, the content of dopamine (DA) and its derivation homovanillic acid (HVA) synthesized by differentiated cells in hypoxia group are significantly higher than normoxia group (P<0.01 and P<0.05 respectively). Conclusion Hypoxia could improve the differentiation of human bone marrow-derived mesenchymal stem cell into dopaminergic neurons; this provides a new clue for clinical treatment of Parkison's Disease with hMSCs.

赵彤李海生（天津）张翠萍靳冰（北京）丁爱石朱玲玲范明. 低氧促进hMSCs 向多巴胺能神经元分化[J]. 基础医学与临床, 2007, 27(4): 377-381.

. Hypoxia enhanced the differentiation of human bone marrow-derived mesenchymal stem cells into dopaminergic neurons[J]. Basic & Clinical Medicine, 2007, 27(4): 377-381.