阿托伐他对THP-1巨噬细胞ATP结合盒转运子A1mRNA表达的影响

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (3): 310-313.

阿托伐他对THP-1巨噬细胞ATP结合盒转运子A1mRNA表达的影响

王秀萍容春莉于薇李如意彭应心齐晓勇

石家庄市河北省人民医院心脏二科河北省人民医院心脏中心河北省人民医院心脏中心河北省人民医院心脏中心河北省人民医院心脏中心河北省人民医院心脏中心

收稿日期:2006-07-14 修回日期:2006-11-16 出版日期:2007-03-25 发布日期:2007-03-25
通讯作者: 王秀萍

Effects of atorvastatin on expression of ATP-binding cassette transporter transporter AI in THP-1 macrophages

Received:2006-07-14 Revised:2006-11-16 Online:2007-03-25 Published:2007-03-25

摘要/Abstract

摘要： 目的探讨阿托伐他汀对THP-1巨噬细胞ATP结合盒转运子A1(ABCA1)mRNA和蛋白及LXRαmRNA 表达的影响。方法体外培养的人THP-1单核细胞,经氟波酯(PMA)诱导48h,使形成巨噬细胞, 将分化良好的巨噬细胞分别加入含不同浓度或不含阿托伐他汀的无血清RPMI 1640培养基，培养24h或48h，收集细胞。用多聚酶链反应（RT-PCR）和免疫组化法检测 THP-1巨噬细胞ABCA1mRNA和蛋白及LXRαmRNA的表达。结果含阿托伐他汀（10μmol/L）的培养基体外培养人THP-1巨噬细胞24h，ABCA1 ( 相对表达量IA值1.21，对照组1.48)和LXRαmRNA （IA值0.87，对照组1.12）表达比对照组明显减少（P<0.05），延长培养时间，ABCA1和LXRαmRNA表达有进一步减少趋势。结论阿托伐他汀抑制体外培养的人THP-1巨噬细胞ABCA1mRNA和蛋白及LXRαmRNA的表达。

Abstract: Objectives:To investigate the effection of atorvastatin on expression of ABCA1 （mRNA，protein） and LXRαmRNA in THP-1 macrophages. Methods: Cultured THP-1 cells were induced to form macrophages by PMA for 48 hours. The macrophages were incubated with atorvastatin in different concention for 24 0r 48 hours. We determined the changes of ABCA1mRNA, protein and LXRαmRNA by reverse trancriptase polymerase chain reaction (RT-PCR) and immuno- histochemistry. Results: The expression levels of ABCA1 mRNA（ratio of relative expression 1.21 vs 1.48） and protein as well as LXRαmRNA(0.87 vs1.12) were decreased in THP-1macrophages when cultured with atorvastatin(10μmol/L) for 24h compared with control. They would be lessen further with the cultured time. Conclusions: Atorvastatin inhibit the expression of ABCA1 mRNA and protein as well as LXRαmRNA of THP-1macrophages in vitro.

王秀萍容春莉于薇李如意彭应心齐晓勇. 阿托伐他对THP-1巨噬细胞ATP结合盒转运子A1mRNA表达的影响[J]. 基础医学与临床, 2007, 27(3): 310-313.

. Effects of atorvastatin on expression of ATP-binding cassette transporter transporter AI in THP-1 macrophages[J]. Basic & Clinical Medicine, 2007, 27(3): 310-313.