大鼠肝纤维化组织信号分子RhoA和肌球蛋白轻链的动态表达

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (3): 275-278.

大鼠肝纤维化组织信号分子RhoA和肌球蛋白轻链的动态表达

王玉珍姜慧卿扈彩霞吕晓萍王丽娜甄承恩

河北省人民医院消化科河北医科大学第二医院消化科河北医科大学第二医院消化内科石家庄河北省人民医院消化科河北省人民医院消化科河北省人民医院消化科

收稿日期:2006-03-06 修回日期:2006-07-14 出版日期:2007-03-25 发布日期:2007-03-25
通讯作者: 姜慧卿

Expression of signaling molecule RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis

Received:2006-03-06 Revised:2006-07-14 Online:2007-03-25 Published:2007-03-25

摘要/Abstract

摘要： 目的观察Rho/Rho激酶信号转导通路关键信号分子RhoA和磷酸化肌球蛋白轻链〔p-MLC(Thr18/Ser19)〕在大鼠肝纤维化组织中的表达规律。方法 HE及Masson三色染色观察肝病理组织学变化；用Western blot 检测RhoA、p-MLC(Thr18/Ser19)蛋白的表达；RT-PCR方法检测RhoA mRNA的表达。结果随着肝纤维化的进展，RhoA、p-MLC(Thr18/Ser19)蛋白表达明显增加， RhoA mRNA基因表达也逐渐加强。RhoA和p-MLC(Thr18/Ser19)分别与α-平滑肌肌动蛋白（α-SMA）呈显著正相关。结论 Rho/Rho激酶信号转导通路在肝纤维化形成过程中发生变化。

Abstract: Objective To observe the expressive changes of RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis. Methods The liver histopathological changes were evaluated by hematoxylin and eosin staining and Masson's trichrome method. Western blot was used to determine the expressions of RhoA and p-MLC(Thr18/Ser19), and reverse transcription-polymerase chain reaction (RT-PCR) to determine the expression of RhoA mRNA. Results With the development of hepatic fibrogenesis, the protein expressions of RhoA and p-MLC(Thr18/Ser19) and the mRNA expression of RhoA were significantly increased. RhoA and p-MLC(Thr18/Ser19)correlated with α-SMA positively, respectively. Conclusions Rho/ROCK signaling pathways are changed during hepatic fibrogenesis.

王玉珍姜慧卿扈彩霞吕晓萍王丽娜甄承恩. 大鼠肝纤维化组织信号分子RhoA和肌球蛋白轻链的动态表达[J]. 基础医学与临床, 2007, 27(3): 275-278.

. Expression of signaling molecule RhoA and phosphorylated myosin light chain in rat liver tissue during hepatic fibrogenesis[J]. Basic & Clinical Medicine, 2007, 27(3): 275-278.