肾上腺髓质素和内皮素在糖尿病大鼠肾脏的表达及氯沙坦的干预研究

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (3): 271-274.

肾上腺髓质素和内皮素在糖尿病大鼠肾脏的表达及氯沙坦的干预研究

高峰（河北）刘青娟段惠军

河北医科大学病理教研室河北医科大学病理教研室河北医科大学病理教研室

收稿日期:2005-11-02 修回日期:2006-08-04 出版日期:2007-03-25 发布日期:2007-03-25
通讯作者: 段惠军

Study on the therapeutic effect losartan on ADM and ET-1 in diabetic rats

Department of Pathology, Hebei Medical University

Received:2005-11-02 Revised:2006-08-04 Online:2007-03-25 Published:2007-03-25

摘要/Abstract

摘要： 目的: 探讨应用血管紧张素Ⅱ（AngⅡ）受体1拮抗剂对糖尿病大鼠肾脏肾上腺髓质素（ADM）、内皮素-1（ET-1）及其各自受体表达的影响。方法单侧切除大鼠肾，在其腹腔内注射STZ，诱发实验性糖尿病模型。随机将大鼠分为：右肾切除对照组（A组）、糖尿病组（B组）和氯沙坦治疗组（C组）。采用免疫组化检测ADM和ET-1的表达，Western blot检测ADMR和EDNR-A蛋白的表达，原位杂交检测ADM mRNA和RT-PCR检测ET-1 mRNA的表达。结果 B组较A组各表达指标均显著增强，C组较B组各表达指标均减低。结论氯沙坦可能通过影响ADM、 ET-1及其各自受体的表达，发挥对肾脏的保护作用。

Abstract: Objective To investigate the therapeutic effect of Losartan on ADM, ET-1 and their receptors in the kidney of diabetic rats. Methods Experimental diabetes were induced in uninephrectomized rats by STZ. The animals were divided into three groups: group A (control group), group B (diabetic group) and group C (Losartan-treated group). Immunohistochemistry, western bloting, in situ hybridyzation and RT-PCR were used to detect the protein and mRNA expressions of ADM, ADMR, ET-1 and EDNR-A. Results Compared with group A, the expressions of ADM, ADMR, ET-1 and EDNR-A in group B distinctly increased. After Losartan treatment, those debased. Conclusions The expressions of ADM, ET-1 and their receptors may be responsible for the renal protective effect of Losartan in diabetic rats.

高峰（河北）刘青娟段惠军. 肾上腺髓质素和内皮素在糖尿病大鼠肾脏的表达及氯沙坦的干预研究[J]. 基础医学与临床, 2007, 27(3): 271-274.

. Study on the therapeutic effect losartan on ADM and ET-1 in diabetic rats[J]. Basic & Clinical Medicine, 2007, 27(3): 271-274.