基础医学与临床 ›› 2007, Vol. 27 ›› Issue (12): 1370-1372.

• 研究论文 • 上一篇    下一篇

丹参酮ⅡA通过阻断MAPK抑制糖尿病大鼠血管平滑肌细胞增殖

李艳平 保春华   

  1. 杭州浙江省中医院检验科 杭州市红十字会医院放射科
  • 收稿日期:2006-12-25 修回日期:2007-06-12 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 李艳平

Effect of TanshinoneⅡA on Diabetic Vascular Complication and its Relationship with MAPK Signal Pathway

Yan-ping LI, Chun-hua BAO   

  1. Clinical Laboratory, Zhejiang Hospital of Chinese Medicine
  • Received:2006-12-25 Revised:2007-06-12 Online:2007-12-25 Published:2007-12-25
  • Contact: Yan-ping LI,

摘要: 目的 研究丹参酮ⅡA(TSN)对糖尿病大鼠血管平滑肌细胞(VSMCs)增殖的作用及与丝裂原活化蛋白激酶(MAPK)信号传导通路的关系。方法 培养糖尿病大鼠血管平滑肌细胞,BrdU掺入DNA的ELISA法测定细胞内DNA合成水平,同位素示踪法测定MAPK活性。结果 原代培养的大鼠VSMCs内MAPK活性较高,使用TSN和U0126后,可明显抑制细胞的增殖和MAPK活性。结论 TSN对糖尿病VSMCs 内DNA的合成具有明显抑制作用,其机制可能是阻断了MAPK信号传导通路。

Abstract: Objective: To study the effect of TanshinoneⅡA (TSN) on vascular smooth muscle cells (VSMCs) induced by high glucose and its relationship with MAPK signal pathway. Methods: After the establishment of the model of diabetic rats, the aortic of rats was separated carefully to culture VSMCs with the method of explant culture. DNA synthesis was analyzed by ELISA of BrdU. The activity of MAPK was measured by liquid scintillation counting. Result: It was shown that TSN inhibited the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats in dose-dependent manner. TSN at the dose of 0.5μg·ml-1 significantly decreased the activity of MAPK, as well as of U0126. Conclusion: The effect of TSN on the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats might be due to the inhibition of MAPK.