基础医学与临床 ›› 2007, Vol. 27 ›› Issue (12): 1309-1313.

• 研究论文 • 上一篇    下一篇

口服卡介菌对过敏性哮喘小鼠的治疗作用

高扬 王立群 周长玉 许潇天 孔祥茜 林嘉友 高歌   

  1. 基础医学研究所 吉林大学中日联谊医院内科 吉林大学中日联谊医院内科 协和医科大学基础医学研究所免疫系 吉林大学中日联谊医院内科 协和医科大学基础医学研究所免疫学系 吉林大学中日联谊医院内科
  • 收稿日期:2007-01-09 修回日期:2007-05-21 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 高歌

Effect of oral BCG on treatment of allergic asthmatic mouse model

Yang GAO, Li-qun WANG, Chang-yu ZHOU, Xiao-tian XU, Xiang-qian KONG, Jia-you LIN, Ge GAO   

  1. Department of immunology, institute of basic medical science, CAMS & PUMC
  • Received:2007-01-09 Revised:2007-05-21 Online:2007-12-25 Published:2007-12-25
  • Contact: Ge GAO

摘要: 目的 探讨口服卡介菌(BCG)治疗Ⅰ型超敏反应的疗效和可能的机制。方法 以OA诱导小鼠过敏性哮喘模型,通过口服途径给药,观察不同剂量BCG对哮喘症状的出现时间,严重程度;ELISA方法和PCA方法检测特异性IgG、IgE抗体滴度;RT-PCR方法检测IL-1、2、10、12 mRNA表达的变化。结果 BCG在0.5~2.0mg/kg剂量范围内,能够减少哮喘小鼠单位时间内的搔鼻次数,推迟攻击后哮喘发作的时间,降低肺气道阻力,较少特异性IgE抗体的生成,调节细胞因子mRNA的表达。结论 口服BCG对治疗过敏性哮喘有效,作用机制可能与特异性抗原口服免疫耐受不同。

Abstract: Objective To explore the effects and possible mechanisms of oral BCG in treating anaphylactic-type I reaction. Methods Ovalbumin sensitized mice were treated with BCG from low-dose to high-dose by oral application and the emergence time, severity of asthma symptoms ,also the effects no the antibody titre of specific IgE and IgG and the mRNA of IL-1,2,10,12 with different doses were recorded. Results Oral BCG range from 0.5mg/kg to 2.0mg/kg can reduce the frequency of nose scraping in allergic mice in a standard times and delay the emergence time of asthma symptoms, reduce the air way resistance and the generation of the antibody titre of specific IgE, and regulate the mRNA expression of cytokins. Conclusions Oral BCG is valid on treatment of allergic asthmatic mouse model.The mechanism of induced tolerance immune were difference with the specific antigen and BCG.