基础医学与临床 ›› 2007, Vol. 27 ›› Issue (12): 1305-1308.

• 研究论文 •    下一篇

C57小鼠肝、肾、脾、胸腺组织时钟基因的表达特点

刘姝 蔡彦宁 谢淑 关云谦 陈彪   

  1. 首都医科大学宣武医院老年病医学研究所神经生物学室 首都医科大学宣武医院神经生物室 首都医科大学宣武医院老年病医学研究所神经生物学室 首都医科大学宣武医院细胞治疗是心 首都医科大学宣武医院神经内科
  • 收稿日期:2007-01-09 修回日期:2007-05-21 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 蔡彦宁

Characters of Clock Gene Expression in C57 Mouse Peripheral Tissues

Shu LIU, Yan-ning CAI, Shu XIE, Yun-qian GUANG, Biao CHEN   

  1. Department of Neurobiology, XuanWu Hospital, Capital University of Medical Science, Key laboratory for neurodegenerative diseases of Ministry of Education
  • Received:2007-01-09 Revised:2007-05-21 Online:2007-12-25 Published:2007-12-25
  • Contact: Yan-ning CAI,

摘要: 目的 探测不同脏器组织中时钟基因的表达水平及其波动性。方法 用实时荧光定量PCR方法检测C57小鼠肝、肾、脾和胸腺中4种重要时钟基因mBMAL1、mPER1、mCRY1和mCRY2的表达。结果 肝和肾中4种时钟基因的表达水平均有显著波动(P<0.01);而脾中仅mBMAL1、mPER1、mCRY1的节律性表达具有统计学意义(P<0.01,<0.001,0.024),并且波动振幅较为平和;胸腺中4种时钟基因的表达均不具备昼夜波动的特征,同时表达水平也与其他3种组织存在显著差异(P<0.01)。时钟基因所构成的环路在不同组织中也不尽相同,mPER1的负反馈效应在肝中较为主要,mCRY1和mCRY2的负反馈效应在肾和脾中较为主要。结论 不同周围组织的分子时钟存在着显著的差异,其相关正负反馈元件和环路有待进一步研究阐明。

Abstract: Objectives To detect temporal expression profiles of 4 principle clock genes (mBMAL1, mPER1, mCRY1 and mCRY2) in liver, kidney, spleen and testis. Methods Real time PCR assays were employed in this study. Results It indicated that cyclic expressions of clock genes in liver and kidney were both robust and significant (P<0.01, respectively). On the other hand, daily fluctuations of mBMAL1, mPER1, mCRY1 were only mild in spleen (P values equal to 0.008, <0.01, 0.024, respectively), where mCRY2 did not show any significant daily variation at all (P>0.05, respectively). In testis, all of these clock genes did not vary with time, and their abundances were different from other tissues significantly. Conclusions Molecular feedback loops seem to work differently in different tissues, with mPER1 working more efficiently in liver and mCRY1/2 in kidney and spleen, which warrants further studies to elucidate tissue dependant clock machinery.