红景天对阿霉素肾病大鼠肾小管间质α-SMA的影响

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (11): 1285-1288.

红景天对阿霉素肾病大鼠肾小管间质α-SMA的影响

张文娟

苏州大学

收稿日期:2006-04-21 修回日期:2006-11-01 出版日期:2007-11-25 发布日期:2007-11-25
通讯作者: 张文娟

The effect of Rhodiola herb on α-SMA of renal tubulointerstitial in rats with adriamycin-induced nephropathy

Received:2006-04-21 Revised:2006-11-01 Online:2007-11-25 Published:2007-11-25

摘要/Abstract

摘要： 目的探讨红景天对阿霉素肾病大鼠肾小管上皮细胞表型转化的作用。方法用阿霉素诱导制成大鼠肾病模型，红景天治疗组第4周起喂服诺迪康胶囊(相当于圣地红景天2.0g生药量/kg.d)，正常对照组及肾病对照组喂水(3ml/kg.d)，于第4、8、12、16、20周末分批处死大鼠，观察肾组织病理变化, 检测肌成纤维细胞(MFB)的标志蛋白—α平滑肌肌动蛋白(α-SMA)在肾小管间质中的表达。结果显示肾病对照组大鼠肾小管间质α-SMA表达增加，半定量分析明显高于红景天治疗组，P<0．01，且与小管间质损害程度相关。结论阿霉素肾病模型中肾小管间质存在细胞表型转化现象，红景天能抑制肾小管间质细胞表型转化，对肾间质的纤维化有较好的防治作用。

关键词: 阿霉素肾病, 圣地红景天, α平滑肌肌动蛋白, 肌成纤维细胞

Abstract: Objective To investigate the effects of Rhodiola herb on renal tubulointerstitial injury in ADR rats．Methods The ADR model was established in rats by tail vein injection of adriamycin 3.0mg／kg twice ．The rats were randomly as signed to normal control group，nephropathy group and Rhodiola herb treatment group．The rats were killed at the time of 4,8,12,16,20 week and to obtain samples．Renal histopathological changes were observed，the expressions ofα-SMA in the constit of the kidney Was examined by utilizing immunohistochemistry. Results Compared with the treatment group，the staining of α-SMA within the tubulonterstitium of nephropathy group was stronger．The expression of in interstitial α-SMA was increased with the degree of tubulointerstitial injury. Conclusion Rhodiola herb has a protective effect on tuhulointerstitial injury in ADR nephropathy，which may realize through the inhibition of epithelial-mesenchymal transition.

Key words: adriamycin-induced nephropathy, Rhodiola herb, α-smooth muscle actin, Myofibroblast

张文娟. 红景天对阿霉素肾病大鼠肾小管间质α-SMA的影响[J]. 基础医学与临床, 2007, 27(11): 1285-1288.

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