p53与妊娠滋养细胞肿瘤的增殖和凋亡

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (11): 1277-1280.

p53与妊娠滋养细胞肿瘤的增殖和凋亡

胡建功敖亚洲谢朝辉宋立刚王宁董雪峰张祥宏

河北医科大学承德医学院附属医院河北省承德市肿瘤医院承德医学院附属医院承德医学院附属医院承德市肿瘤医院河北医科大学基础研究所实验病理室石家庄

收稿日期:2007-01-19 修回日期:2007-03-13 出版日期:2007-11-25 发布日期:2007-11-25
通讯作者: 谢朝辉

The Correlation between the Expression of p53 and Proliferation, Apoptosis In Gestational Trophoblastic Tumor

Received:2007-01-19 Revised:2007-03-13 Online:2007-11-25 Published:2007-11-25

摘要/Abstract

摘要： [摘要] 目的:探讨p53与妊娠滋养细胞肿瘤（GTT）中细胞增殖、凋亡的关系。方法:采用免疫组化S-P法及核酸原位末端标记（TUNEL）法，检测了10例正常早期绒毛（NP）、10例完全性葡萄胎（CM）、20例侵袭性葡萄胎（IM）、19例绒癌（CCA）中的p53和PCNA的表达及细胞凋亡。结果: 在NP、CM、IM 和CCA中，p53表达率（p53-I）分别为4.12%、21.68%、39.61%、27.39%，各组间差异有统计学意义(F=6.687, P=0.001)；PCNA表达率（PI）分别为10.40%、20.76%、53.60%、51.95%，(F=17.510, P=0.000)；凋亡指数（AI）分别为1.88%、2.59%、6.45%、1.26%，各组间差异有统计学意义，(F=7.020, P=0.000)。p53-I和PI呈显著正相关（r=0.587，P=0.000），而与AI无明确的相关性（r=0.113，P=0.096）。结论: GTT的发生是由于机体监控能力减弱或消失、滋养细胞过度增生及增生与凋亡调节系统失调所致；p53能促进GTT中的细胞增殖活性，与GTT的发生发展密切相关；未发现p53与GTT的细胞凋亡相关性。

Abstract: Abstract Objective: To elucidate the features of the expression of p53, proliferation and apoptosis in gestational trophoblastic tumor (GTT). Methods: 10 cases of normal placenta, 10 cases of complete hydatidiform mole (CM), 20 cases of invasive hydatidiform mole (IM) and 19 cases of choriocarcinoma(CCA) paraffin-embedded tissues were studied. P53 and PCNA were detected with immunohistochemistry and apoptosis was detected with terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). Results: In NP, CM, IM and CCA, The p53 index(p53-I) in them were 4.12%, 21.68%, 39.61% and 27.39% respectively with significant differences among them (F=6.687, P=0.001). the PCNA index (PI) were 10.40%, 20.76%, 53.60% and 51.95% respectively with significant differences among them (F=17.510, P=0.000). The Apoptosis index (AI) in them were 1.88%, 2.59%, 6.45% and 1.26% respectively with significant differences among them (F=7.020, P=0.000). Significantly positive correlation was found between p53-I and PI（r=0.587，P=0.000）, while there is no correlation between p53-I and AI（r=0.113，P=0.096）. Conclusion: The process of GTT is associated with trophoblastic cell overproliferation because the proliferation and apoptosis are out of control. The increasing expression of muta-p53 contributes to the cell proliferation in GTT and can be helpful to the progress of GTT. No correlation was found between p53-I and AI.

胡建功敖亚洲谢朝辉宋立刚王宁董雪峰张祥宏. p53与妊娠滋养细胞肿瘤的增殖和凋亡[J]. 基础医学与临床, 2007, 27(11): 1277-1280.

. The Correlation between the Expression of p53 and Proliferation, Apoptosis In Gestational Trophoblastic Tumor[J]. Basic & Clinical Medicine, 2007, 27(11): 1277-1280.