雅培CD3700测定白细胞线性范围的验证与ISO15189应用

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (10): 1165-1167.

雅培CD3700测定白细胞线性范围的验证与ISO15189应用

杨有业杨志钊缪丽韶黄福达

中山市人民医院中山市人民医院中山市人民医院中山市人民医院

收稿日期:2007-07-20 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25
通讯作者: 杨有业

Certification of the linear range of WBC measured by CD3700 and Application in ISO15189

Received:2007-07-20 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 目的验证雅培CD3700血细胞计数仪测定血液白细胞的线性范围。方法用浓缩白膜层制备高值白细胞标本，以生理盐水稀释成系列浓度标本，每个浓度测定2次，各浓度的测定顺序随机，对数据进行离群值检验、回归分析和随机误差分析。结果测定结果无离群值，经回归检验在（0～219）×109L-1范围内为一条通过0点的直线，直线的随机误差小于1/4CLIA'88允许误差范围(3.75%)。结论 CD3700测定血液白细胞的线性范围与该型号仪器出厂标准一致。

Abstract: Objective To certify the linear range of WBC measured by CD3700. Method Use the white stratum of blood after centrifugation as high value specimen. Then, the high value specimens were diluted with physiological saline to get a series of samples with different concentrations. Every specimen was measured twice in order to keep the sequence of specimens randomly. The perform outlier test, polynomial regression analysis, and the random error estimation were carried out. Results There was no outlier in the results. It was demonstrated by polynomial regression to be linear from （0 ~ 219）×109 L-1. The random error was within the allowable limit of 1/4CLIA'88(3.75%). Conclusion The linear range of WBC measured by CD3700 is just like that of the manufacturer claims.

杨有业杨志钊缪丽韶黄福达. 雅培CD3700测定白细胞线性范围的验证与ISO15189应用[J]. 基础医学与临床, 2007, 27(10): 1165-1167.

. Certification of the linear range of WBC measured by CD3700 and Application in ISO15189[J]. Basic & Clinical Medicine, 2007, 27(10): 1165-1167.